Qi Lin, Zhang Yang
Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, Liaoning, China.
Tumour Biol. 2014 Apr;35(4):3139-44. doi: 10.1007/s13277-013-1410-y. Epub 2013 Nov 20.
In these studies, inhibitor of growth protein 5 (ING5) and various fragments of it were overexpressed in the human tongue squamous cell carcinoma cell line, HSC-3. The roles of ING5 in HSC-3 cells were then identified in vitro. Our results indicate that intact ING5 can inhibit proliferation and induce apoptosis in HSC-3 cells. Moreover, two truncated fragments of ING5 (aa 1-184 and aa 107-226) can induce cellular senescence. To analyze the signaling pathway involved, western blotting was performed. In these assays, two truncated fragments of ING5 were found to inhibit the cyclin E and CDK2 expression. These results are consistent with the S phase arrest observed with the overexpression of truncated ING5. However, the mechanisms of ING5-induced cellular senescence remain unclear, and extensive investigations are required in future studies.
在这些研究中，生长抑制蛋白5（ING5）及其各种片段在人舌鳞状细胞癌细胞系HSC-3中过表达。然后在体外确定ING5在HSC-3细胞中的作用。我们的结果表明，完整的ING5可以抑制HSC-3细胞的增殖并诱导其凋亡。此外，ING5的两个截短片段（氨基酸1-184和氨基酸107-226）可诱导细胞衰老。为了分析所涉及的信号通路，进行了蛋白质印迹分析。在这些试验中，发现ING5的两个截短片段可抑制细胞周期蛋白E和CDK2的表达。这些结果与截短的ING5过表达所观察到的S期阻滞一致。然而，ING5诱导细胞衰老的机制仍不清楚，未来的研究需要进行广泛的调查。
