• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

血液谷胱甘肽过氧化物酶:一种测量方法以及储存、氰化物和 Drabkin 试剂对酶活性的影响。

Blood glutathione peroxidase : A method for measurement and the influence of storage, cyanide, and Drabkin's reagent on enzyme activity.

机构信息

Department of Agriculture, Veterinary Research Laboratories, Stoney Road, BT4 3SD, Belfast, Northern Ireland.

出版信息

Biol Trace Elem Res. 1986 Dec;11(1):89-100. doi: 10.1007/BF02795527.

DOI:10.1007/BF02795527
PMID:24254506
Abstract

Manual and automated assays for the determination of glutathione peroxidase activity in bovine, sheep, pig, and human blood samples are described. The reactivation of the enzyme in blood samples after storage at -20°C was examined. Cyanide and Drabkin's reagent, as used to prepare hemolysates in other assays, have been shown to inhibit enzyme activity, particularly in stored blood samples, and is omitted from this assay.

摘要

本文描述了用于测定牛、羊、猪和人血样中谷胱甘肽过氧化物酶活性的手动和自动检测方法。研究了血样在-20°C 储存后酶的再激活情况。氰化物和 Drabkin 试剂(用于其他检测方法制备溶血物)已被证明会抑制酶活性,特别是在储存的血样中,因此本检测方法中省略了这两种试剂。

相似文献

1
Blood glutathione peroxidase : A method for measurement and the influence of storage, cyanide, and Drabkin's reagent on enzyme activity.血液谷胱甘肽过氧化物酶:一种测量方法以及储存、氰化物和 Drabkin 试剂对酶活性的影响。
Biol Trace Elem Res. 1986 Dec;11(1):89-100. doi: 10.1007/BF02795527.
2
Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods.采用血红蛋白比色法和沙利氏法对血红蛋白进行的对比研究。
J Postgrad Med. 1992 Jan-Mar;38(1):8-9.
3
A comparative study of common techniques used to measure haemolysis in stored red cell concentrates.储存红细胞制剂中常用的溶血检测技术比较研究。
Vox Sang. 2010 Feb;98(2):116-23. doi: 10.1111/j.1423-0410.2009.01249.x. Epub 2009 Aug 30.
4
The stability of glutathione peroxidase activity in plasma from cattle, pigs and sheep on storage in the presence and absence of glutathione.在有和没有谷胱甘肽存在的情况下,牛、猪和羊血浆中谷胱甘肽过氧化物酶活性在储存时的稳定性。
Vet Res Commun. 1990;14(6):441-6. doi: 10.1007/BF00367055.
5
Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity.用于测定谷胱甘肽过氧化物酶活性的肝素化犬全血的储存
Biol Trace Elem Res. 2016 Aug;172(2):361-363. doi: 10.1007/s12011-015-0603-1. Epub 2015 Dec 23.
6
A new reagent for the rapid determination of total hemoglobin as hemiglobincyanide in blood containing carboxyhemoglobin.一种用于快速测定含碳氧血红蛋白血液中总血红蛋白(以氰化高铁血红蛋白形式)的新型试剂。
Biochem Med. 1983 Aug;30(1):78-88. doi: 10.1016/0006-2944(83)90010-8.
7
[Structure of cyanmethemoglobin and determination of hemospharin according to Drabkin's method].[氰化高铁血红蛋白的结构及根据德拉布金法测定肝素]
Delt Hell Mikrobiol Hygieinol Hetair. 1957 May-Jun;2(3):192-6.
8
Stability of glutathione peroxidase in swine plasma samples under various storage conditions.谷胱甘肽过氧化物酶在不同储存条件下猪血浆样本中的稳定性。
Can J Vet Res. 1986 Jul;50(3):390-2.
9
An analysis of the bias in red blood cell hemolysis measurement using several analytical approaches.几种分析方法评估红细胞溶血测量中的偏倚。
Clin Chim Acta. 2012 Nov 12;413(21-22):1746-52. doi: 10.1016/j.cca.2012.06.028. Epub 2012 Jun 29.
10
New approaches in tail-bleeding assay in mice: improving an important method for designing new anti-thrombotic agents.小鼠尾部出血试验的新方法:改进设计新型抗血栓药物的重要方法
Int J Exp Pathol. 2016 Jun;97(3):285-92. doi: 10.1111/iep.12182. Epub 2016 Jul 5.

引用本文的文献

1
The stability of glutathione peroxidase activity in plasma from cattle, pigs and sheep on storage in the presence and absence of glutathione.在有和没有谷胱甘肽存在的情况下,牛、猪和羊血浆中谷胱甘肽过氧化物酶活性在储存时的稳定性。
Vet Res Commun. 1990;14(6):441-6. doi: 10.1007/BF00367055.
2
Oxidant defense mechanisms in the human colon.人类结肠中的氧化防御机制。
Inflammation. 1990 Dec;14(6):669-80. doi: 10.1007/BF00916370.

本文引用的文献

1
The selenium status of Belgian population groups : I. Healthy adults.比利时人群的硒状况:I. 健康成年人。
Biol Trace Elem Res. 1983 Apr;5(2):91-102. doi: 10.1007/BF02916629.
2
Effect of selenium deficiency on liver and blood glutathione peroxidase activity in guinea pigs.硒缺乏对豚鼠肝脏和血液中谷胱甘肽过氧化物酶活性的影响。
J Nutr. 1981 Apr;111(4):690-3. doi: 10.1093/jn/111.4.690.
3
Procedure for blood glutathione peroxidase determination in cattle and swine.牛和猪血液谷胱甘肽过氧化物酶测定方法
Acta Vet Scand. 1982;23(4):515-27. doi: 10.1186/BF03546770.
4
Effect of prolonged supplementation with daily supplements of selenomethionine and sodium selenite on glutathione peroxidase activity in blood of New Zealand residents.每日补充硒代蛋氨酸和亚硒酸钠对新西兰居民血液中谷胱甘肽过氧化物酶活性的长期影响。
Am J Clin Nutr. 1982 Jul;36(1):24-31. doi: 10.1093/ajcn/36.1.24.
5
Blood selenium and glutathione peroxidase levels and dietary selenium of free-living and institutionalized elderly subjects.自由生活和机构养老老年人的血硒、谷胱甘肽过氧化物酶水平及膳食硒情况
Proc Soc Exp Biol Med. 1983 May;173(1):87-95. doi: 10.3181/00379727-173-41614.
6
An automated analysis of glutathione peroxidase, S-transferase, and reductase activity in animal tissue.动物组织中谷胱甘肽过氧化物酶、S-转移酶和还原酶活性的自动化分析。
J Anal Toxicol. 1983 Mar-Apr;7(2):86-8. doi: 10.1093/jat/7.2.86.
7
Blood selenium levels and glutathione-peroxidase activities in university and chronic intravenous hyperalimentation subjects.
Proc Soc Exp Biol Med. 1981 Jul;167(3):383-90. doi: 10.3181/00379727-167-41184.
8
Comparison of selenium levels and glutathione peroxidase activity in bovine whole blood.牛全血中硒水平与谷胱甘肽过氧化物酶活性的比较。
Can J Comp Med. 1984 Oct;48(4):431-3.
9
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.红细胞谷胱甘肽过氧化物酶的定量和定性特征研究。
J Lab Clin Med. 1967 Jul;70(1):158-69.
10
Selenium as a component of glutathione periodase isolated from ovine erythrocytes.从绵羊红细胞中分离出的谷胱甘肽过氧化物酶中的一种成分——硒。
Biochemistry. 1974 Apr 23;13(9):1825-9. doi: 10.1021/bi00706a008.