血液谷胱甘肽过氧化物酶：一种测量方法以及储存、氰化物和 Drabkin 试剂对酶活性的影响。
Blood glutathione peroxidase : A method for measurement and the influence of storage, cyanide, and Drabkin's reagent on enzyme activity.
机构信息
Department of Agriculture, Veterinary Research Laboratories, Stoney Road, BT4 3SD, Belfast, Northern Ireland.
出版信息
Biol Trace Elem Res. 1986 Dec;11(1):89-100. doi: 10.1007/BF02795527.
PMID:24254506
Abstract
Manual and automated assays for the determination of glutathione peroxidase activity in bovine, sheep, pig, and human blood samples are described. The reactivation of the enzyme in blood samples after storage at -20°C was examined. Cyanide and Drabkin's reagent, as used to prepare hemolysates in other assays, have been shown to inhibit enzyme activity, particularly in stored blood samples, and is omitted from this assay.
摘要
本文描述了用于测定牛、羊、猪和人血样中谷胱甘肽过氧化物酶活性的手动和自动检测方法。研究了血样在-20°C 储存后酶的再激活情况。氰化物和 Drabkin 试剂（用于其他检测方法制备溶血物）已被证明会抑制酶活性，特别是在储存的血样中，因此本检测方法中省略了这两种试剂。
Zotero 插件