Nuñez G, Stastny P
Tissue Antigens. 1986 Apr;27(4):185-95. doi: 10.1111/j.1399-0039.1986.tb01519.x.
We have used a lysostripping technique to investigate the molecular relationships of the determinants recognized by human alloantisera and murine monoclonal antibodies reactive with HLA-D region products. Cytotoxicity mediated by sera that define the specificities DR1, DR2, DR3, DR4, DR7, DRw11, DRw12, DRw13, DRw14, DRw52 and DRw53 was inhibited by anti-DR but not by anti-DQ Mabs. In contrast, sera that define DQw1, DQw2 and DQw3 were inhibited by anti-DQ but not anti-DR monoclonal antibodies. We found some sera used to define DR specificities that were not inhibited by monoclonal anti-DR. Cytotoxicity mediated by monoclonal antibodies reactive with the recently described specificities TA10 and MC1 was inhibited by anti-DQ and anti-DR monoclonal antibodies, respectively. The lysostripping technique is useful for the assignment of specific determinants to HLA-D subregion molecules.
我们运用了溶酶体剥离技术来研究人类同种异体抗血清及与HLA - D区产物反应的鼠单克隆抗体所识别的决定簇之间的分子关系。定义DR1、DR2、DR3、DR4、DR7、DRw11、DRw12、DRw13、DRw14、DRw52和DRw53特异性的血清所介导的细胞毒性被抗DR单克隆抗体抑制，但不被抗DQ单克隆抗体抑制。相反，定义DQw1、DQw2和DQw3的血清被抗DQ单克隆抗体抑制，但不被抗DR单克隆抗体抑制。我们发现一些用于定义DR特异性的血清不被单克隆抗DR抑制。与最近描述的TA10和MC1特异性反应的单克隆抗体所介导的细胞毒性分别被抗DQ和抗DR单克隆抗体抑制。溶酶体剥离技术有助于将特定决定簇指定给HLA - D亚区分子。
