Myers L K, Ball E J, Nuñez G, Stastny P
Immunogenetics. 1986;23(3):142-50. doi: 10.1007/BF00373814.
The HLA-D region of individuals with the DRw11, w52, DQw3 haplotype encodes multiple molecular products of three distinct subregions, DR, DP, and DQ. Since each molecule can carry multiple stimulatory epitopes, the repertoire of allogeneic T-cell responses to determinants of this haplotype can be quite large. In the present experiments, alloreactive cloned T-cell lines recognized six distinct epitopes associated with DRw11, DRw52, DQw3 haplotypes. Panel studies established that three epitopes were DRw11-like and three were DRw52-like. Blocking with monoclonal antibodies showed that two DRw11-like epitopes were carried by DR-subregion products and one DRw11-like epitope was carried by DQ-subregion molecules. DRw52-like epitopes were detected on separate DR subregion-encoded molecules. One of them carried both DRw11- and DRw52-like epitopes, the other carried two of the DRw52-like epitopes. These epitopes, which represent functional units that trigger T-cell responses, can be detected at the present time only with the methods used in this report. Conventional allogeneic T-cell responses represent the summation of responses to multiple epitopes encoded by different D-subregion genes.
具有DRw11、w52、DQw3单倍型的个体的HLA - D区域编码DR、DP和DQ这三个不同亚区域的多种分子产物。由于每个分子都可以携带多个刺激表位，因此针对该单倍型决定簇的同种异体T细胞反应库可能相当大。在本实验中，同种异体反应性克隆T细胞系识别出与DRw11、DRw52、DQw3单倍型相关的六个不同表位。分组研究确定，三个表位类似DRw11，三个表位类似DRw52。用单克隆抗体阻断显示，两个类似DRw11的表位由DR亚区域产物携带，一个类似DRw11的表位由DQ亚区域分子携带。在单独的DR亚区域编码分子上检测到类似DRw52的表位。其中一个携带类似DRw11和DRw52的表位，另一个携带两个类似DRw52的表位。这些表位代表触发T细胞反应的功能单位，目前只能用本报告中使用的方法检测到。传统的同种异体T细胞反应代表对不同D亚区域基因编码的多个表位的反应总和。
