Department of Medical Physics, Austin Hospital, Melbourne.
Biol Trace Elem Res. 1987 Apr;12(1):143. doi: 10.1007/BF02796672.
Sampling, storage, and preparation of biological material for analysis are some of the most important factors in the study of trace elements in biomedical fields. If due care is not given to these factors, the resulting data may often be meaningless, regardless of the analytical technique chosen for analysis. A survey of the data in the literature would support this view, in which wide differences in the trace element composition of biological tissues have been observed, and not all these differences are attributed to variations in the biological systems.In this paper, the following points are discussed in detail: (A) Sampling and Storage Sampling methods and program. Contamination of the sample during sampling and storage. Changes in the composition during storage. Various storage methods for solid and liquid samples. (B) Sample Preparation Drying and ashing. Wet digestion. Different methods for preparing samples of hard, semihard, and soft tissues, and of biological fluids.
生物材料的采样、储存和制备是生物医学领域微量元素研究中最重要的因素之一。如果不注意这些因素，即使选择了分析技术进行分析，所得数据也常常是无意义的。对文献中的数据进行调查可以支持这一观点，因为在生物组织的微量元素组成中观察到了广泛的差异，而且并非所有这些差异都归因于生物系统的变化。在本文中，详细讨论了以下几点：(A)采样和储存 采样方法和程序。采样和储存过程中的样品污染。储存过程中组成的变化。固态和液态样品的各种储存方法。(B)样品制备 干燥和灰化。湿法消解。硬组织、半硬组织和软组织以及生物体液样品的不同制备方法。
