Department of Physical Biology, Gustaf Werner Institute, Uppsala University, Box 531, S-751 21, Uppsala, Sweden.
Biol Trace Elem Res. 1987 Apr;12(1):351-62. doi: 10.1007/BF02796691.
Trace elements are indispensable for the effective and proper functioning of biological systems. Recent years have demonstrated the conspicuous lack of knowledge about trace-element physiology. Establishment of reference values is a very difficult task, requiring the consideration of and compensation for a number of possible simultaneous phenomena.Peripheral blood has been used in medical diagnosis for a very long time, because, among other things, it is easily accessible. In the search for signs of lack or excess of minerals and trace elements in disease, the interest has been focused mainly on blood plasma or serum. The utilization of blood cells as a marker model is proposed here. The advent of the nuclear microprobe made possible the determination of elemental profiles of individual cells.The techniques of blood cell separation and preparation for microprobe analysis are presented and discussed. As an example of a possible diagnostic application, a set of reference data from a control group is compared to corresponding data from a group of patients suffering from acute myeloid leukemia.
微量元素对于生物系统的有效和正常功能是不可或缺的。近年来，人们明显缺乏对微量元素生理学的认识。建立参考值是一项非常困难的任务，需要考虑并补偿许多可能同时发生的现象。外周血在医学诊断中已经使用了很长时间，因为它不仅容易获得，而且还可以提供有关身体内部状态的重要信息。在寻找疾病中矿物质和微量元素缺乏或过量的迹象时，主要关注的是血浆或血清。本文提出了将血细胞用作标记模型。核微探针的出现使得确定单个细胞的元素分布成为可能。本文介绍并讨论了血细胞分离和准备用于微探针分析的技术。作为可能的诊断应用的一个例子，将对照组的一组参考数据与患有急性髓细胞性白血病的一组患者的相应数据进行了比较。
