Mulware Stephen Juma
Louisiana Accelerator Center, Department of Physics, University of Louisiana at Lafayette, Broussard Hall No. 115, Lafayette, LA 70504, USA.
J Biophys. 2015;2015:740751. doi: 10.1155/2015/740751. Epub 2015 Nov 18.
The properties of many biological materials often depend on the spatial distribution and concentration of the trace elements present in a matrix. Scientists have over the years tried various techniques including classical physical and chemical analyzing techniques each with relative level of accuracy. However, with the development of spatially sensitive submicron beams, the nuclear microprobe techniques using focused proton beams for the elemental analysis of biological materials have yielded significant success. In this paper, the basic principles of the commonly used microprobe techniques of STIM, RBS, and PIXE for trace elemental analysis are discussed. The details for sample preparation, the detection, and data collection and analysis are discussed. Finally, an application of the techniques to analysis of corn roots for elemental distribution and concentration is presented.
许多生物材料的特性往往取决于基质中微量元素的空间分布和浓度。多年来，科学家们尝试了各种技术，包括经典的物理和化学分析技术，每种技术都有相对的准确度。然而，随着空间敏感亚微米束的发展，使用聚焦质子束对生物材料进行元素分析的核微探针技术取得了显著成功。本文讨论了用于痕量元素分析的常用微探针技术STIM、RBS和PIXE的基本原理。还讨论了样品制备、检测以及数据收集和分析的细节。最后，介绍了这些技术在分析玉米根中元素分布和浓度方面的应用。
