Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.
PLoS One. 2013 Nov 8;8(11):e79838. doi: 10.1371/journal.pone.0079838. eCollection 2013.
To investigate the effect of phenolic environmental estrogens on uterine leiomyoma from the perspective of clinical epidemiology.
Urine and blood samples were collected from Han women with uterine leiomyoma and women without uterine leiomyoma, living in Nanjing, China, between September 2011 and February 2013. A total of 156 urine samples and 214 blood samples were collected from the uterine leiomyoma group and 106 urine samples and 126 blood plasma samples from the control group. Bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) concentrations were determined by solid-phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
Phenolic environmental estrogens in the uterine leiomyoma and control groups were compared based on: gravida>3 and gravida ≤ 3. In participants with gravida>3, urine OP concentration was significantly (P<0.05) higher in the uterine leiomyoma group than in the control group. In participants with gravida ≤ 3, urine NP concentration was significantly (P<0.05) higher in the uterine leiomyoma group compared to controls. Despite obstetric history, urine BPA mean exposure concentration was significantly (P<0.05) different between uterine leiomyoma group and control group. The urine BPA concentration was not significantly (P>0.05) different between gravida>3 and gravida ≤ 3 patients. There was no significant (P>0.05) difference in plasma concentrations of BPA, OP and NP between the leiomyoma group and control group. Mean exposure concentration and range of distribution of BPA, OP and NP plasma concentration differed between the uterine leiomyoma and control group.
Exposure level of phenolic environmental estrogens in human was related with leiomyoma tumorigenesis.
从临床流行病学的角度探讨酚类环境雌激素对子宫肌瘤的影响。
2011 年 9 月至 2013 年 2 月，采集中国南京子宫肌瘤和非子宫肌瘤女性的尿液和血液样本。子宫肌瘤组共采集 156 份尿液和 214 份血液样本，对照组采集 106 份尿液和 126 份血浆样本。采用固相萃取（SPE）结合液相色谱-串联质谱（HPLC-MS/MS）法测定尿液中的双酚 A（BPA）、壬基酚（NP）和辛基酚（OP）浓度。
比较了子宫肌瘤组和对照组的生育史>3 次和生育史≤3 次患者的酚类环境雌激素水平。生育史>3 次的患者中，子宫肌瘤组尿液 OP 浓度明显高于对照组（P<0.05）。生育史≤3 次的患者中，子宫肌瘤组尿液 NP 浓度明显高于对照组（P<0.05）。无论生育史如何，子宫肌瘤组和对照组的尿液 BPA 平均暴露浓度均有显著差异（P<0.05）。生育史>3 次和生育史≤3 次患者的尿液 BPA 浓度无显著差异（P>0.05）。子宫肌瘤组和对照组患者的血浆 BPA、OP 和 NP 浓度无显著差异（P>0.05）。子宫肌瘤组和对照组的 BPA、OP 和 NP 血浆浓度的平均暴露浓度和分布范围存在差异。
酚类环境雌激素的暴露水平与子宫肌瘤的发生有关。
