Xia Anne, Stempak Joanne M, Grist Jesse, Bressler Brian, Silverberg Mark S, Bach Horacio
Division of Infectious Diseases, Department of Medicine, University of British Columbia , Vancouver, BC , Canada.
Scand J Gastroenterol. 2014 Feb;49(2):157-63. doi: 10.3109/00365521.2013.857713. Epub 2013 Nov 21.
The link between Mycobacterium avium subsp. paratuberculosis (MAP) and Crohn's disease (CD) is supported by several studies that have reported the detection and isolation of MAP from human tissues, but causation has not yet been proven. Preliminary studies have shown higher levels of antibodies in sera from CD patients against secreted protein from MAP within human macrophages when compared to healthy controls. The immunogenicity of this protein in CD patients under different treatment regimes was evaluated.
Sera of 110 CD patients, 82 ulcerative colitis (UC), and 150 healthy controls were collected and the presence of antibodies against the mycobacterial protein tyrosine phosphatase PtpA was assayed using ELISA.
A statistically significant difference in the level of antibodies against PtpA was measured in untreated CD patients versus healthy controls, but variation in the antibody levels was observed when patients were subjected to different treatment regimens. UC patients showed no differences in the levels of antibodies against PtpA when compared to healthy controls.
CD patients under different drug treatments show a clear difference in the levels of antibodies against a protein secreted by MAP, suggesting that if MAP is active in the progress of CD, some treatments can be detrimental to its survival.
多项研究报告了从人体组织中检测和分离出鸟分枝杆菌副结核亚种（MAP），这支持了MAP与克罗恩病（CD）之间的联系，但因果关系尚未得到证实。初步研究表明，与健康对照相比，CD患者血清中针对人巨噬细胞内MAP分泌蛋白的抗体水平更高。评估了该蛋白在不同治疗方案下的CD患者中的免疫原性。
收集110例CD患者、82例溃疡性结肠炎（UC）患者和150例健康对照的血清，采用酶联免疫吸附测定法检测抗分枝杆菌蛋白酪氨酸磷酸酶PtpA抗体的存在情况。
未经治疗的CD患者与健康对照相比，抗PtpA抗体水平存在统计学显著差异，但当患者接受不同治疗方案时，抗体水平存在变化。与健康对照相比，UC患者抗PtpA抗体水平无差异。
接受不同药物治疗的CD患者在抗MAP分泌蛋白的抗体水平上存在明显差异，这表明如果MAP在CD进展中具有活性，某些治疗可能对其生存不利。
