*Département de Biochimie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, rue Jean Mignault, Sherbrooke, QC, Canada, J1E 4K8.
Biochem Soc Trans. 2013 Dec;41(6):1679-86. doi: 10.1042/BST2013089.
The fission yeast Schizosaccharomyces pombe has been successfully used as a model to gain fundamental knowledge in understanding how eukaryotic cells acquire copper during vegetative growth. These studies have revealed the existence of a heteromeric Ctr4-Ctr5 plasma membrane complex that mediates uptake of copper within the cells. Furthermore, additional studies have led to the identification of one of the first vacuolar copper transporters, Ctr6, as well as the copper-responsive Cuf1 transcription factor. Recent investigations have extended the use of S. pombe to elucidate new roles for copper metabolism in meiotic differentiation. For example, these studies have led to the discovery of Mfc1, which turned out to be the first example of a meiosis-specific copper transporter. Whereas copper-dependent transcriptional regulation of the Ctr family members is under the control of Cuf1 during mitosis or meiosis, meiosis-specific copper transporter Mfc1 is regulated by the recently discovered transactivator Mca1. It is foreseeable that identification of novel meiotic copper-related proteins will serve as stepping stones to unravel fundamental aspects of copper homoeostasis.
裂殖酵母（Schizosaccharomyces pombe）已成功用作模型，以获得有关真核细胞在营养生长过程中获取铜的基本认识。这些研究揭示了存在一种异源 Ctr4-Ctr5 质膜复合物，该复合物介导细胞内铜的摄取。此外，进一步的研究导致鉴定了第一个液泡铜转运蛋白 Ctr6 以及铜响应的 Cuf1 转录因子。最近的调查将裂殖酵母的用途扩展到阐明铜代谢在减数分裂分化中的新作用。例如，这些研究导致发现了 Mfc1，它原来是第一个减数分裂特异性铜转运蛋白的例子。在有丝分裂或减数分裂期间，Ctr 家族成员的铜依赖性转录调控受 Cuf1 控制，而减数分裂特异性铜转运蛋白 Mfc1 则受最近发现的转录激活子 Mca1 调控。可以预见的是，鉴定新的减数分裂铜相关蛋白将成为揭示铜动态平衡基本方面的踏脚石。
