毛茛科翠雀属植物中的二萜生物碱和黄酮类化合物。

Diterpenoid alkaloids and flavonoids from Delphinium trichophorum.

机构信息

Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.

School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.

出版信息

Phytochemistry. 2014 Jan;97:88-95. doi: 10.1016/j.phytochem.2013.10.011. Epub 2013 Nov 17.

DOI:10.1016/j.phytochem.2013.10.011

PMID:24256579

Abstract

Five hetisane-type C20-diterpenoid alkaloids, trichodelphinines A-E, one delnudine-type C20-diterpenoid alkaloid, trichodelphinine F and three known flavonoids, quercetin, quercetin 3-O-β-D-glucopyranoside, and quercetin 3-O-β-D-glucopyranoside-7-O-α-L-arabinopyranoside, were isolated from whole plants of Delphinium trichophorum Franch. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HSQC, HMBC, (1)H-(1)H COSY, NOESY and X-ray crystallographic analysis, and from chemical evidence. The cytotoxic activities of the diterpenoid alkaloids were evaluated using the MTT method, and the IC50 values of their cytotoxicity against A549 cancer cells ranged from 12.03 to 52.79 μM.

摘要

从蓝盆花属植物全草中分离得到了 5 种 hetisane 型 C20-二萜生物碱，即 Trichodelphinines A-E，1 种 delnudine 型 C20-二萜生物碱，即 Trichodelphinine F，以及 3 种已知的黄酮类化合物，槲皮素、槲皮素 3-O-β-D-吡喃葡萄糖苷和槲皮素 3-O-β-D-吡喃葡萄糖苷-7-O-α-L-阿拉伯吡喃糖苷。基于广泛的光谱分析，包括 HSQC、HMBC、(1)H-(1)H COSY、NOESY 和 X 射线晶体学分析，以及化学证据，确定了它们的结构。采用 MTT 法评价了二萜生物碱的细胞毒性，其对 A549 癌细胞的半数抑制浓度（IC50）值范围为 12.03 至 52.79 μM。