Stutts M J, Schwab J H, Chen M G, Knowles M R, Boucher R C
Am Rev Respir Dis. 1986 Jul;134(1):17-21. doi: 10.1164/arrd.1986.134.1.17.
During chronic lung infections the effects of bacterial exoproducts on the function of the airways epithelium could play a role in the overall pathophysiology of infection. We measured the effects of Pseudomonas aeruginosa culture on ion transport and solute permeability of dog bronchial epithelium to determine if this organism produces toxins that affect these epithelial functions. Thirty-six-hour trypticase soy broth cultures of P. aeruginosa at 1:40 dilutions decreased short-circuit current and active sodium absorption and lowered conductance and chloride unidirectional fluxes. At 1:4 dilutions, P. aeruginosa increased conductance and the permeability coefficient of 14C-mannitol, suggesting that solute flow around cells was increased. These effects appear to be caused by a heat-stable glycolipid hemolysin produced by P. aeruginosa.
在慢性肺部感染期间，细菌外产物对气道上皮功能的影响可能在感染的整体病理生理学中起作用。我们测量了铜绿假单胞菌培养物对犬支气管上皮离子转运和溶质通透性的影响，以确定该微生物是否产生影响这些上皮功能的毒素。铜绿假单胞菌在1:40稀释度下培养36小时的胰蛋白酶大豆肉汤培养物降低了短路电流和活性钠吸收，并降低了电导和氯离子单向通量。在1:4稀释度下，铜绿假单胞菌增加了电导和14C-甘露醇的通透系数，表明细胞周围的溶质流动增加。这些影响似乎是由铜绿假单胞菌产生的热稳定糖脂溶血素引起的。
