Lemberger Stephanie I K, Deeg Cornelia A, Hauck Stefanie M, Amann Barbara, Hirmer Sieglinde, Hartmann Katrin, Dorsch Roswitha
Clinic of Small Animal Medicine, Faculty of Veterinary Medicine, Ludwig Maximilian University, 80539 Munich, Germany.
Am J Vet Res. 2011 Oct;72(10):1407-15. doi: 10.2460/ajvr.72.10.1407.
To characterize and compare the urine protein content in cats without urinary tract disease and cats with idiopathic cystitis (IdC), bacterial urinary tract infection (UTI), or urolithiasis.
Control cats (n = 18) and cats with IdC (18), UTI (12), and urolithiasis (12) from which urine samples were obtained and 2 cats with obstructive IdC and 4 additional control cats from which postmortem urinary bladder biopsy specimens were obtained.
Protein contents in urine samples obtained via cystocentesis or catheterization were measured via the Bradford method. Urine proteins were separated by means of 1-dimensional gel electrophoresis. Evaluation of fibronectin content was performed via western blotting and immunohistochemical analysis. Urinary bladder biopsy specimens were examined histologically and analyzed immunohistochemically for fibronectin.
Urine fibronectin content was significantly greater in cats with IdC, compared with control cat findings. Urine fibronectin contents did not differ significantly among controls and cats with UTI or urolithiasis. Histologic examination of bladder biopsy specimens obtained from 2 cats with obstructive IdC revealed destruction of the urothelial lining of the urinary bladder and severe fibrosis; immunohistochemical analysis revealed few fluorescence signals for fibronectin, unlike findings in control bladder biopsy specimens.
Results indicated that urine fibronectin content in cats with IdC was greater than that in controls, cats with UTI, or cats with urolithiasis. In cats with IdC, increased permeability of damaged urothelium may result in detachment and leakage of fibronectin into urine. Urine fibronectin might serve as a biomarker for diagnosis of IdC in cats.
对无泌尿道疾病的猫与患有特发性膀胱炎（IdC）、细菌性尿路感染（UTI）或尿石症的猫的尿蛋白含量进行特征描述和比较。
采集尿液样本的对照猫（n = 18）以及患有IdC（18只）、UTI（12只）和尿石症（12只）的猫，还有2只患有梗阻性IdC的猫和另外4只对照猫，从这些猫身上获取了死后膀胱活检标本。
通过膀胱穿刺术或导尿术获取的尿液样本中的蛋白质含量采用Bradford法进行测量。尿蛋白通过一维凝胶电泳进行分离。通过蛋白质印迹法和免疫组织化学分析对纤连蛋白含量进行评估。对膀胱活检标本进行组织学检查，并对纤连蛋白进行免疫组织化学分析。
与对照猫的结果相比，患有IdC的猫尿纤连蛋白含量显著更高。对照猫与患有UTI或尿石症的猫之间尿纤连蛋白含量无显著差异。对2只患有梗阻性IdC的猫的膀胱活检标本进行组织学检查，发现膀胱尿路上皮衬里破坏和严重纤维化；免疫组织化学分析显示纤连蛋白的荧光信号很少，这与对照膀胱活检标本的结果不同。
结果表明，患有IdC的猫的尿纤连蛋白含量高于对照猫、患有UTI的猫或患有尿石症的猫。在患有IdC的猫中，受损尿路上皮通透性增加可能导致纤连蛋白脱离并漏入尿液。尿纤连蛋白可能作为猫IdC诊断的生物标志物。
