腺苷脱氨酶可作为二肽基肽酶 4 介导的中东呼吸综合征冠状病毒进入的天然拮抗剂。
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
机构信息
Department of Viroscience, Erasmus Medical Center, Rotterdam, the Netherlands.
出版信息
J Virol. 2014 Feb;88(3):1834-8. doi: 10.1128/JVI.02935-13. Epub 2013 Nov 20.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection.
摘要
中东呼吸综合征冠状病毒（MERS-CoV）使用二肽基肽酶 4（DPP4）作为功能性受体在不同物种的细胞中复制。在这里，我们展示了雪貂对 MERS-CoV 感染的抵抗力，以及雪貂 DPP4 无法结合 MERS-CoV 的事实。因此，对氨基酸可变的雪貂 DPP4 进行定点突变，揭示了功能性人 DPP4 病毒结合位点。腺苷脱氨酶（ADA），一种 DPP4 结合蛋白，与病毒竞争结合，作为 MERS-CoV 感染的天然拮抗剂。
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