van Doremalen Neeltje, Miazgowicz Kerri L, Munster Vincent J
Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA
J Virol. 2016 May 12;90(11):5499-5502. doi: 10.1128/JVI.03267-15. Print 2016 Jun 1.
The novel emerging coronavirus Middle East respiratory syndrome coronavirus (MERS-CoV) binds to its receptor, dipeptidyl peptidase 4 (DPP4), via 14 interacting amino acids. We previously showed that if the five interacting amino acids which differ between hamster and human DPP4 are changed to the residues found in human DPP4, hamster DPP4 does act as a receptor. Here, we show that the functionality of hamster DPP4 as a receptor is severely decreased if less than 4 out of 5 amino acids are changed.
The novel emerging coronavirus MERS-CoV has infected >1,600 people worldwide, and the case fatality rate is ∼36%. In this study, we show that by changing 4 amino acids in hamster DPP4, this protein functions as a receptor for MERS-CoV. This work is vital in the development of new small-animal models, which will broaden our understanding of MERS-CoV and be instrumental in the development of countermeasures.
新型冠状病毒中东呼吸综合征冠状病毒（MERS-CoV）通过14个相互作用的氨基酸与其受体二肽基肽酶4（DPP4）结合。我们之前表明，如果将仓鼠和人类DPP4之间不同的五个相互作用氨基酸替换为人类DPP4中的残基，仓鼠DPP4确实会充当受体。在此，我们表明，如果五个氨基酸中少于4个被改变，仓鼠DPP4作为受体的功能会严重降低。
新型冠状病毒MERS-CoV已在全球感染了1600多人，病死率约为36%。在本研究中，我们表明，通过改变仓鼠DPP4中的4个氨基酸，该蛋白可作为MERS-CoV的受体。这项工作对于开发新的小动物模型至关重要，这将拓宽我们对MERS-CoV的理解，并有助于开发应对措施。
