噬菌体 mv4 位点特异性重组：P2 mv4Int 结合位点的核心作用。
Bacteriophage mv4 site-specific recombination: the central role of the P2 mv4Int-binding site.
机构信息
Université de Toulouse, Université Paul Sabatier, Laboratoire de Microbiologie et de Génétique Moléculaires, Toulouse, France.
出版信息
J Virol. 2014 Feb;88(3):1839-42. doi: 10.1128/JVI.02735-13. Epub 2013 Nov 20.
Abstract
The contributions of the five (mv4)Int- and two (mv4)Xis arm-binding sites to the spatial intasome organization of bacteriophage mv4 were found not to be equivalent. The 8-bp overlap region was mapped to the left extremity of the core region and is directly flanked by the P2 Int arm-binding site. These results and the absence of characteristic Int core-binding sites suggest that the P2 site is the determinant for integrase positioning and recognition of the core region.
摘要
发现五（mv4）个 Int-和两个（mv4）Xis 臂结合位点对噬菌体 mv4 的空间整合体组织的贡献并不相等。8 个碱基对的重叠区域被映射到核心区域的左侧末端，并且直接由 P2 Int 臂结合位点侧翼。这些结果以及缺乏特征性的 Int 核心结合位点表明，P2 位点是整合酶定位和识别核心区域的决定因素。
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