Biology Department, The Open University, Walton Hall, MK7 6AA, Milton Keynes, UK.
Plant Cell Rep. 1982 Oct;1(5):229-31. doi: 10.1007/BF00270243.
An improved technique is described for the culture of explants from Helianthus tuberosus L. (Jerusalem artichoke). No xylem is formed when tuber discs are pre-cultured on a medium containing ßNAA, allowing uninfected discs to be selected for investigation of xylogenesis. Subsequent growth on a medium containing 0.45μM 2,4-D and 9.3μM kinetin stimulates a high proportion (up to 36%) of the cells to differentiate into xylem elements within a relatively short time (between 1 and 3 d after transfer).
介绍了一种改良的方法，用于培养菊芋（洋姜）的外植体。当块茎圆盘在含有 ßNAA 的培养基上预培养时，不会形成木质部，这使得可以选择未感染的圆盘来研究木质部的形成。随后在含有 0.45μM 2,4-D 和 9.3μM 激动素的培养基上生长，在相对较短的时间内（转移后 1 至 3 天内），刺激较高比例（高达 36%）的细胞分化为木质部元素。
