Department of Botany and Microbiology, University College London, Gower Street, WCIE 6BT, London, UK.
Planta. 1977 Jan;135(3):207-12. doi: 10.1007/BF00384891.
the culture of Jerusalem artichoke (Helianthus tuberosus L.) tuber explants on filter paper discs moistened with liquid medium resulted in rapid and consistent xylem differentiation. The number of tracheary elements increased in discrete steps, the first at 48 h with a second at 56-58 h, following partially synchronous mitoses at 20 and 30 h. Factors favouring xylem cell differentiation were optimum levels of both an auxin and a cytokinin, low medium nitrogen concentrations, small volumes of medium, and high culture temperatures. A cell counting method employing Feulgen-stained nuclei and suitable for quantifyings small numbers of immature tracheary elements is described.
在湿润的滤纸圆盘上培养菊芋（Helianthus tuberosus L.）外植体，可快速且稳定地诱导木质部分化。导管分子的数量呈离散阶跃式增加，第一次在 48 小时，第二次在 56-58 小时，随后在 20 和 30 小时经历部分同步有丝分裂。有利于木质部细胞分化的因素是适宜水平的生长素和细胞分裂素、低浓度的培养基氮、小体积的培养基和较高的培养温度。本文描述了一种细胞计数方法，该方法采用 Feulgen 染色的核，可以定量分析少量未成熟的导管分子。
