Phillips R, Arnott S M
Histochem J. 1983 May;15(5):427-36. doi: 10.1007/BF01002697.
A model system for the study of cell differentiation in plants is described. Tracheary elements differentiate from storage parenchyma cells in cultured explants of the Jerusalem artichoke, Helianthus tuberosus, in the presence of auxin and cytokinin. Differentiation occurs in G1 cells following three successive divisions of precursor cells; the onset of secondary wall deposition follows within 6--8 h of the final mitosis. Differentiation is inhibited by levels of FUdR, actinomycin-D, cycloheximide or gamma-irradiation sufficient to block mitosis. Inhibition was equally effective whether blocking treatments were applied at the beginning of the culture period or after one or two rounds of mitosis.
本文描述了一种用于研究植物细胞分化的模型系统。在生长素和细胞分裂素存在的情况下，管状分子从菊芋（Helianthus tuberosus）培养外植体中的贮藏薄壁细胞分化而来。分化发生在前体细胞连续三次分裂后的G1期细胞中；最终有丝分裂后6 - 8小时内开始次生壁沉积。足以阻断有丝分裂的氟尿嘧啶核苷（FUdR）、放线菌素D、环己酰亚胺或γ射线照射水平会抑制分化。无论在培养期开始时还是在一轮或两轮有丝分裂后进行阻断处理，抑制效果均相同。
