School of Agricultural Engineering, Jiangsu University, Zhenjiang, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
Arch Microbiol. 2021 Jul;203(5):2681-2687. doi: 10.1007/s00203-021-02201-0. Epub 2021 Mar 14.
A Gram-staining negative, motile, non-spore-forming, rod-shaped bacterium, designated NAJP-14, was isolated from the alkali-saline soil in Heilongjiang, Northeast China. Phylogenetic analysis based on 16S rRNA gene sequencing illustrated that strain NAJP-14 was a member of the genus Pelagibacterium, and shared 94.6-96.6% sequence identities to species from the genus Pelagibacterium. Strain NAJP-14 grew at 20-45 °C (optimum, 30 °C), pH 7.0-10.0 (optimum, pH 8.0) and in the presence of up to 5% w/v NaCl. The menaquinone was determined to be Q (10). The major fatty acids were identified as Cw6c (38.7%), C (16.2%) and C cyclo w8c (13.9%). The G + C content of the genomic DNA was 61.2%. Out of the 3442 predicted genes, 3391 were protein-coding genes and 51 were ncRNA. Digital DNA-DNA hybridization (dDDH) estimation and average nucleotide identity (ANI) of the strain NAJP-14 and the type strains of related species in the same family ranged between 17.9 and 21.8% and between 61.4 and 78.7%, respectively. Based on these data, it is concluded that strain NAJP-14 possesses sufficient characteristics to differentiate it from all recognized Pelagibacterium species, and should be considered as a novel species for which the name Pelagibacterium limicola sp. nov. is proposed. The type strain is NAJP-14 (= CGMCC 1.16631, = JCM 33746).
一株革兰氏染色阴性、运动、非孢子形成、杆状细菌，被命名为 NAJP-14，从中国东北黑龙江的盐碱土壤中分离出来。基于 16S rRNA 基因测序的系统发育分析表明，菌株 NAJP-14 是 Pelagibacterium 属的一个成员，与 Pelagibacterium 属的种具有 94.6-96.6%的序列同一性。菌株 NAJP-14 在 20-45°C（最佳温度 30°C）、pH 值 7.0-10.0（最佳 pH 值 8.0）和高达 5%w/v NaCl 的条件下生长。鉴定出的menaquinone 为 Q (10)。主要脂肪酸为 Cw6c（38.7%）、C（16.2%）和 C 环 w8c（13.9%）。基因组 DNA 的 G+C 含量为 61.2%。在 3442 个预测基因中，有 3391 个是编码蛋白的基因，有 51 个是 ncRNA。该菌株 NAJP-14 与同一家族的相关种的模式菌株之间的数字 DNA-DNA 杂交（dDDH）估计值和平均核苷酸同一性（ANI）分别为 17.9-21.8%和 61.4-78.7%。基于这些数据，可以得出结论，菌株 NAJP-14 具有足够的特征，可以将其与所有已识别的 Pelagibacterium 种区分开来，应被视为一个新种，建议命名为 Limicola pelagibacterium 种。其模式菌株为 NAJP-14（=CGMCC 1.16631，=JCM 33746）。
