School of Life Sciences, Liaoning University, No. 66 Chongshan Middle Road, Huanggu District, Shenyang, 110036, People's Republic of China.
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
Arch Microbiol. 2022 Jul 2;204(7):449. doi: 10.1007/s00203-022-03036-z.
A Gram-positive, motile, rod-shaped and lignin-degrading novel actinomycete, designated strain NEAU-YY56, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, Central China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-YY56 belonged to the genus Cellulomonas and exhibited 16S rRNA gene sequence similarities of 98.7, 98.2 and 98.1% to Cellulomonas pakistanensis JCM 18755, Cellulomonas denverensis JCM 14733 and Cellulomonas hominis JCM 12133, respectively. The whole-cell sugars were glucose, rhamnose and ribose. The peptidoglycan of strain NEAU-YY56 contained ornithine and glutamic acid. The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and two unknown glycolipids. The major menaquinone was MK-9(H). The major fatty acids (> 5.0%) were identified as anteiso-C, C, C and anteiso-C. Meanwhile, DNA G+C content was 74.7%. The morphological and chemotaxonomic properties of strain NEAU-YY56 were also confirmed the affiliation of the isolate to the genus Cellulomonas. However, physiological and biochemical characteristics indicated that strain NEAU-YY56 can be clearly differentiated from its closest relatives. In addition, the ANI values and dDDH levels between strain NEAU-YY56 and related Cellulomonas species were lower than the accepted threshold value. Therefore, it is concluded that strain NEAU-YY56 represents a novel species of the genus Cellulomonas, for which the name Cellulomonas triticagri sp. nov. is proposed. The type strain is NEAU-YY56 (= DSM 106717 = JCM 32550).
一株革兰氏阳性、运动性、杆状、能降解木质素的新型放线菌，被命名为 NEAU-YY56 菌株，从中国中部河南省驻马店采集的小麦（Triticum aestivum L.）根际土壤中分离得到，并采用多相分类法进行了表征。基于 16S rRNA 基因序列的系统发育分析表明，NEAU-YY56 菌株属于纤维单胞菌属，与 Cellulomonas pakistanensis JCM 18755、Cellulomonas denverensis JCM 14733 和 Cellulomonas hominis JCM 12133 的 16S rRNA 基因序列相似性分别为 98.7%、98.2%和 98.1%。该菌株的全细胞糖为葡萄糖、鼠李糖和核糖。其肽聚糖含有鸟氨酸和谷氨酸。磷脂图谱中含有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷和两种未知的糖脂。主要的menaquinone 为 MK-9(H)。主要脂肪酸（>5.0%）为 anteiso-C15:0、C16:0、C17:0 和 anteiso-C17:0。同时，DNA G+C 含量为 74.7%。NEAU-YY56 菌株的形态学和化学分类特征也证实了该分离株与纤维单胞菌属的归属。然而，生理生化特征表明，NEAU-YY56 菌株可以与最接近的亲缘关系明显区分开来。此外，NEAU-YY56 菌株与相关纤维单胞菌属种之间的 ANI 值和 dDDH 水平均低于可接受的阈值。因此，认为 NEAU-YY56 菌株代表纤维单胞菌属的一个新种，将其命名为 Cellulomonas triticagri sp. nov.。其模式菌株为 NEAU-YY56（=DSM 106717=JCM 32550）。
