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无叶绿素柑橘类质体中叶绿素酶的活性。

Chlorophyllase activity in chlorophyll-free citrus chromoplasts.

机构信息

Department of Horticulture, Faculty of Agriculture, The Hebrew University of Jerusalem, 76100, Rehovot, Israel.

出版信息

Plant Cell Rep. 1983 Jun;2(3):117-8. doi: 10.1007/BF00269332.

DOI:10.1007/BF00269332
PMID:24257977
Abstract

Chromoplast fractions from mature, chlorophyll-less 'Valencia' orange (Citrus sinensis L. Osbeck) flavedo (= the outer coloured layer of citrus peel) showed considerable chlorophyllase activity. Acetone powders prepared from chromoplast fractions had 2.5× higher specific activity than those prepared from whole flavedo. Exposure of mature, chlorophyll-less fruit to ethylene caused a 2.5 to 4.0 fold increase in chlorophyllase activity. Juice chromoplasts showed negligible chlorophyllase activity. The results suggest that chlorophyllase activity as well as its induction by ethylene are not dependent upon the presence of chlorophyll in the tissue.

摘要

成熟、不含叶绿素的“瓦伦西亚”甜橙(Citrus sinensis L. Osbeck)的质体部分显示出相当高的叶绿素酶活性。与整个果皮相比,从质体部分制备的丙酮粉具有高 2.5 倍的比活性。将成熟、不含叶绿素的果实暴露于乙烯中会使叶绿素酶活性增加 2.5 至 4.0 倍。果汁质体几乎没有叶绿素酶活性。结果表明,叶绿素酶活性及其对乙烯的诱导与组织中叶绿素的存在无关。

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引用本文的文献

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Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: a study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization.乙烯诱导果实转色时柑橘叶绿素酶的动态变化:叶绿素酶表达、翻译后加工动力学及原位细胞内定位的研究
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2
Chlorophyllase is a rate-limiting enzyme in chlorophyll catabolism and is posttranslationally regulated.叶绿素酶是叶绿素分解代谢中的一种限速酶,并且受到翻译后调控。
Plant Cell. 2007 Mar;19(3):1007-22. doi: 10.1105/tpc.107.050633. Epub 2007 Mar 16.

本文引用的文献

1
Chlorophyll synthetase in chlorophyll-free chromoplasts.无叶绿素质体中的叶绿素合成酶。
Plant Cell Rep. 1981 Aug;1(1):40-2. doi: 10.1007/BF00267657.
2
Involvement of ethylene in chlorophyll degradation in peel of citrus fruits.乙烯参与柑橘果皮中叶绿素的降解。
Plant Physiol. 1981 Oct;68(4):854-6. doi: 10.1104/pp.68.4.854.
3
Purification and Properties of Chlorophyllase from Ailanthus altissima (Tree-of-Heaven).臭椿叶片叶绿素酶的纯化与性质
Plant Physiol. 1971 May;47(5):609-18. doi: 10.1104/pp.47.5.609.
4
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
5
Influence of lecithin liposomes on chlorophyllase-catalyzed chlorophyll hydrolysis: comparison of intramembraneous and solubilized Phaeodactylum chlorophyllase.卵磷脂脂质体对叶绿素酶催化叶绿素水解的影响:膜内和可溶的三角褐指藻叶绿素酶的比较
Biochim Biophys Acta. 1980 Jul 16;600(1):36-47. doi: 10.1016/0005-2736(80)90409-5.
6
Turnover of the glycerolipids of pumpkin leaves. The importence of phosphatidylcholine.南瓜叶甘油脂质的周转。磷脂酰胆碱的重要性。 需注意,原文中“importence”拼写错误,正确拼写应为“importance”。
Biochem J. 1970 Mar;117(1):1-8. doi: 10.1042/bj1170001.