高粱植株从铝选择培养基再生。

Sorghum plant regeneration from aluminum selection media.

机构信息

Department of Plant Sciences, Texas A&M University, 77843, College Station, TX, USA.

出版信息

Plant Cell Rep. 1983 Jun;2(3):129-32. doi: 10.1007/BF00269336.

Abstract

Callus cultures of four Sorghum bicolor (L.) Moench cultivars were initiated from aseptically germinated seed. Prior to culture on media containing 0, 100, 200, and 400 μM aluminum, the callus had been subcultured for 3 to 12 months. There were differences amongst the cultivars in response to the aluminum in terms of callus growth. Growth was inhibited at the highest level of aluminum tested. Subculture of callus from aluminum selection medium on both aluminum-containing and aluminum-free media indicated that aluminum-selected callus grew better in the presence of aluminum. Plants were regenerated from only one cultivar (IS3620C of the Margaritiferum group) after several passages on aluminum-supplemented media. Regeneration capacity was however inhibited at 400 μM aluminum.

摘要

从无菌发芽的种子中诱导出四种高粱（ Sorghum bicolor （L.） Moench ）品种的愈伤组织培养物。在培养在含有 0、100、200 和 400μM 铝的培养基上之前，愈伤组织已经经过 3 到 12 个月的继代培养。在对铝的反应方面，不同品种的愈伤组织存在差异。在测试的最高铝浓度下，生长受到抑制。从铝选择培养基上的愈伤组织在含有铝和不含铝的培养基上的继代培养表明，铝选择的愈伤组织在铝存在的情况下生长得更好。只有一个品种（Margaritiferum 组的 IS3620C）在添加铝的培养基上经过几次传代后才能再生出植株。然而，在 400μM 铝的存在下，再生能力受到抑制。