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紫外线-C 对花生愈伤组织中芪类化合物形成的作用。

Action of ultraviolet-C on stilbene formation in callus ofArachis hypogaea.

机构信息

Biochemie (Fachbereich Chemie), Philipps-Universität, Hans-Meerwein-Strasse, D-3500, Marburg, Federal Republic of Germany.

出版信息

Planta. 1983 Jan;159(1):25-9. doi: 10.1007/BF00998810.

DOI:10.1007/BF00998810
PMID:24258082
Abstract

The action of light on the formation of stilbenes and the induction of stilbene synthase in dark-grown and light-grown callus of peanut (Arachis hypogaea) was investigated over the wavelength range from 250 to 400 nm. Ultraviolet light of 260-270 nm had a significant and selective effect on the formation of resveratrol and isopentenylresveratrol. The callus responded by the production of stilbene synthase, with maximal activity appearing 4 h after irradiation with a fluence rate of 1 W m(-2) (270 nm) applied for 10 min. At lower fluence rates, maximal responses in enzyme activity were shifted to longer induction periods. The efficiency of the biosynthetic pathway, and the form and maxima of enzyme profiles depended on the duration of exposure. We failed to demonstrate any significant influence of red light at low energy irradiation (672 nm, 726 nm and 753 nm).

摘要

研究了波长范围为 250nm 至 400nm 的光对花生(Arachis hypogaea)黑暗和光照培养愈伤组织中芪类化合物的形成和芪合酶诱导的作用。260nm 至 270nm 的紫外光对白藜芦醇和异戊烯基白藜芦醇的形成有显著的选择性影响。愈伤组织通过产生芪合酶来做出反应,在以 1W m(-2) 的辐照度(270nm)照射 10 分钟后,4 小时后出现最大活性。在较低的辐照度下,酶活性的最大反应被转移到更长的诱导期。生物合成途径的效率、酶谱的形式和最大值取决于暴露时间。我们未能证明低能量照射(672nm、726nm 和 753nm)的红光有任何显著影响。

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本文引用的文献

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Planta. 1981 Jan;151(1):48-52. doi: 10.1007/BF00384236.
2
Involvement of phytochrome and a blue light photoreceptor in UV-B induced flavonoid synthesis in parsley (Petroselinum hortense Hoffm.) cell suspension cultures.UV-B 诱导的欧芹(Petroselinum hortense Hoffm.)细胞悬浮培养物中类黄酮合成涉及光敏色素和蓝光受体。
Planta. 1982 Dec;156(3):213-7. doi: 10.1007/BF00393727.
3
Cultured cells of Arachis hypogaea susceptible to induction of stilbene synthase (resveratrol-forming).
Plants (Basel). 2021 Jan 4;10(1):90. doi: 10.3390/plants10010090.
4
Control of gene expression during induction of cultured peanut cells: mRNA levels, protein synthesis and enzyme activity of stilbene synthase.培养花生细胞诱导过程中基因表达的调控:芪合酶的 mRNA 水平、蛋白质合成和酶活性。
Plant Mol Biol. 1988 May;10(3):235-43. doi: 10.1007/BF00027400.
5
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6
Differential regulation of genes for resveratrol synthase in cell cultures ofArachis hypogaea L.花生细胞培养中白藜芦醇合酶基因的差异调控
Planta. 1990 May;181(2):169-75. doi: 10.1007/BF02411534.
7
Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).葡萄(Vitis vinifera L.)中参与类黄酮和芪生物合成的结构基因的克隆与分子分析。
Plant Mol Biol. 1994 Mar;24(5):743-55. doi: 10.1007/BF00029856.
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