Qayyum Zuhra, Noureen Fatima, Khan Maryam, Khan Marrium, Haider Ghulam, Munir Faiza, Gul Alvina, Amir Rabia
Department of Plant Biotechnology, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad 44000, Pakistan.
Plants (Basel). 2022 Jul 5;11(13):1776. doi: 10.3390/plants11131776.
is an important enzyme of the phenylpropanoid pathway, regulating the production of several biologically active stilbenoids. These compounds have antioxidant, anti-inflammatory, and anti-cancer properties. However, the detailed characterization of genes in has not yet been performed. In this study, the comprehensive characterization of genes in was conducted, commencing with identification, phylogenetic analysis, and study of their expression in response to exogenous hormonal treatment. We identified and isolated five genes and recorded their expression pattern in peanut (BARD-479) in response to methyl jasmonate (MeJA) and salicylic acid (SA) treatment. The presence of Chal_sti_synt, ACP_syn_III, and FAE1_CUT1_rppA domains in all indicated their role in the biosynthesis of stilbene and lipid metabolism. Cis-regulatory element analysis indicated their role in light responsiveness, defense responses, regulation of seed development, plant growth, and development. Despite close structural and functional similarities, expression and correlational analysis suggested that these genes may have a specific role in peanut, as individual exhibited differential expression upon hormonal treatment in a genotype dependent manner. Further studies on functional characterization involving the transcriptional regulation of can clearly explain the differential expression of stilbene synthase genes to hormonal treatment.
是苯丙烷类途径中的一种重要酶，调节几种生物活性芪类化合物的产生。这些化合物具有抗氧化、抗炎和抗癌特性。然而，尚未对[物种名称]中的[基因名称]基因进行详细表征。在本研究中，对[物种名称]中的[基因名称]基因进行了全面表征，首先进行了鉴定、系统发育分析，并研究了它们对外源激素处理的响应表达。我们鉴定并分离了五个[基因名称]基因，并记录了它们在花生（BARD - 479）中对茉莉酸甲酯（MeJA）和水杨酸（SA）处理的表达模式。所有[基因名称]中Chal_sti_synt、ACP_syn_III和FAE1_CUT1_rppA结构域的存在表明它们在芪生物合成和脂质代谢中的作用。顺式调控元件分析表明它们在光响应、防御反应、种子发育调控、植物生长和发育中的作用。尽管结构和功能相似，但表达和相关性分析表明这些基因在花生中可能具有特定作用，因为单个[基因名称]在激素处理后以基因型依赖的方式表现出差异表达。对涉及[基因名称]转录调控的功能表征的进一步研究可以清楚地解释芪合酶基因对激素处理的差异表达。
