Botanisches Institut der Universität, Menzingerstr. 67, D-8000, München 19, FRG.
Plant Cell Rep. 1983 Oct;2(5):261-4. doi: 10.1007/BF00269156.
Somatic embryogenesis in anise (Pimpinella anisum L.) suspension cultures induced by transfer to hormone-free growth medium may be synchronized by previous selection of cell aggregates with diameters between 100-240 μm. Around 80-90% of the embryoids are globular after 2-3 d, heart-shaped after 5-7 d and torpedo-shaped after 9 d. In embryogenic medium without source of carbon or with 20 mmol/l acetate differentiation and growth cease. But like in dedifferentiated cell aggregates the key enzyme activities for glyoxysomes such as isocitrate lyase and malate synthase are induced in globular (3 d old) and heart-shaped (5 d old) embryoids, but not in embryoids at day 7 or later. Similarly, in explants from anise hypocotyl glyoxysomes cannot be derepressed by such treatment. It is concluded that during differentiation of heart-shaped embryoids to torpedo forms the competence of the cells for the yet unknown inducing principle for glyoxysomes is lost.
悬浮培养的茴香体细胞胚胎发生可以通过转移到无激素生长培养基中进行同步化，先前可以选择直径在 100-240μm 之间的细胞聚集体。在 2-3 天后，大约 80-90%的胚状体呈球形，在 5-7 天后呈心形，在 9 天后呈鱼雷形。在没有碳源的胚胎发生培养基或含有 20mmol/L 醋酸盐的培养基中，分化和生长停止。但是，就像在去分化的细胞聚集体中一样，球形（3 天龄）和心形（5 天龄）胚状体中诱导了乙醛酸体的关键酶活性，如异柠檬酸裂解酶和苹果酸合酶，但在 7 天龄或更晚的胚状体中没有诱导。同样，在茴香下胚轴的外植体中，这种处理不能使乙醛酸体去阻遏。因此可以得出结论，在心形胚状体分化为鱼雷形的过程中，细胞丧失了对未知的乙醛酸体诱导因子的敏感性。
