Radeck W, Roebruck P, Schmutzler W
Arzneimittelforschung. 1986 May;36(5):881-3.
In the present study it was investigated if monovalent dextran (Dx 1, HR 573, Promit) possibly involving activated complement is capable of inducing mediator (histamine) release from isolated human mast cells. Dx 1 in the absence or in the presence of 20% fresh human serum did not induce a histamine release from human mast cells. The "non-release" could be established statistically using a new sequential test which provides a substantial reduction of the average sample size required to reach a prescribed test power. In contrast, concanavalin A (Con A) or ionophore A 23187 caused a marked histamine release in the absence of human serum. In the presence of human serum the spontaneous histamine release was increased but the Con A- or ionophore-induced release was inhibited.
在本研究中，对单价葡聚糖（Dx 1，HR 573，Promit）是否可能涉及激活补体从而诱导人离体肥大细胞释放介质（组胺）进行了研究。在不存在或存在20%新鲜人血清的情况下，Dx 1均未诱导人肥大细胞释放组胺。使用一种新的序贯检验可从统计学上确定“无释放”情况，该检验可大幅减少达到规定检验效能所需的平均样本量。相比之下，伴刀豆球蛋白A（Con A）或离子载体A 23187在不存在人血清的情况下可引起显著的组胺释放。在存在人血清的情况下，组胺的自发释放增加，但Con A或离子载体诱导的释放受到抑制。
