Department of Plant Sciences, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843.
Plant Physiol. 1980 May;65(5):944-8. doi: 10.1104/pp.65.5.944.
Antiserum was prepared against nitrate reductase (EC 1.6.6.1) purified to homogeneity from Chlorella vulgaris Beijerinck. Both crude antiserum and anti-nitrate reductase antibodies prepared from it were used as re-agents to study the synthesis of nitrate reductase. Cell extracts from cultures which were grown with ammonia salts as the sole source of nitrogen contained almost no active enzyme. These extracts did contain material which binds to antibody and is thus immunologically related to purified nitrate reductase. The presence of this cross reacting material in cell extracts was detected by the ability of these extracts to (a) lower the titer of antisera; (b) form a biphasic precipitin curve with purified antibody; and (c) increase the peak height of a standard amount of purified nitrate reductase in rocket immunoelectrophoresis assay. These results suggest that ammonia-grown cells contain nitrate reductase precursor protein.
抗血清是针对从普通小球藻中纯化到均一性的硝酸还原酶（EC 1.6.6.1）制备的。粗抗血清和从其制备的抗硝酸还原酶抗体都被用作研究硝酸还原酶合成的试剂。以氨盐作为唯一氮源生长的培养物的细胞提取物几乎不含活性酶。这些提取物确实含有与抗体结合的物质，因此与纯化的硝酸还原酶在免疫学上有关。通过这些提取物能够：（a）降低抗血清的效价；（b）与纯化的抗体形成双相沉淀曲线；以及（c）增加火箭免疫电泳测定中标准量纯化硝酸还原酶的峰高，来检测细胞提取物中这种交叉反应物质的存在。这些结果表明，以氨盐生长的细胞含有硝酸还原酶前体蛋白。
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