Department of Internal Medicine, Division of Endocrinology and Metabolism, Medical University of Graz, Stiftingtalstrasse 24, 8010, Graz, Austria,
Anal Bioanal Chem. 2014 Jan;406(2):549-54. doi: 10.1007/s00216-013-7493-x. Epub 2013 Nov 21.
We report a novel approach to quantify interstitial analytes in living tissue by combining open-flow microperfusion (OFM) with a sensor and the re-circulation method. OFM is based on the unrestricted exchange of molecules between the interstitial fluid (ISF) and a perfusion medium through macroscopic perforations that enables direct access to the ISF. By re-circulating the perfusate and monitoring the changes of the analytes' concentration with a sensor, the absolute analyte concentration in the ISF can be calculated. In order to validate the new concept, the absolute electrical conductivity of the ISF was identified in six subjects to be 1.33 ± 0.08 S/m (coefficient of variation CV = 6 %), showing the robustness of this approach. The most striking feature of this procedure is the possibility to monitor several compounds simultaneously by applying different sensors which will allow not only the determination of the concentration of a single substance in vivo but also the simultaneous dynamics of different analytes. This will open new fields in analytical chemistry, pharmacology, as well as clinical experimental research.
我们报告了一种通过结合开放式微灌注（OFM）与传感器和再循环方法来定量活体组织中细胞间分析物的新方法。OFM 基于分子在间质液（ISF）和灌注介质之间不受限制的交换，通过宏观穿孔实现直接进入 ISF。通过再循环灌注液并使用传感器监测分析物浓度的变化，可以计算出 ISF 中分析物的绝对浓度。为了验证新概念，在六个对象中确定了 ISF 的绝对电导率为 1.33 ± 0.08 S/m（变异系数 CV = 6%），显示了该方法的稳健性。该方法最显著的特点是能够通过应用不同的传感器同时监测多种化合物，这不仅允许在体内确定单个物质的浓度，还允许同时监测不同分析物的动力学。这将为分析化学、药理学以及临床实验研究开辟新的领域。
