Trajanoski Z, Brunner G A, Schaupp L, Ellmerer M, Wach P, Pieber T R, Kotanko P, Skrabal F
Department of Biophysics, Institute of Biomedical Engineering, Graz University of Technology, Austria.
Diabetes Care. 1997 Jul;20(7):1114-21. doi: 10.2337/diacare.20.7.1114.
To evaluate a novel technique for on-line continuous glucose measurement in subcutaneous adipose tissue, and to investigate its accuracy for detection of hypoglycemia.
The method combined an open-flow microperfusion of subcutaneous adipose tissue using a double lumen catheter and an extracorporeal sensor cell. An isotonic ion-free solution was perfused through the inner lumen of the catheter, equilibrated with the subcutaneous tissue fluid, and sampled through the outer lumen. The recovery was continuously monitored as the ratio between the measured sampled fluid conductivity and the subcutaneous tissue fluid conductivity (assumed to have a constant value of 1.28 S/m at 25 degrees C). Glucose concentration was calculated on-line from the measured glucose in the sampled fluid and the measured recovery in healthy volunteers during hyperglycemic glucose loads (n = 8), hypoglycemic hyperinsulinemic clamp (n = 6), and a 24-h monitoring period (n = 7).
Subcutaneous glucose concentrations in the fasting state were 94% of the plasma glucose concentrations in arterialized venous samples. According to the error grid analysis, 96.9% of the on-line measured subcutaneous glucose concentrations during hyperglycemia and 96.3% during hypoglycemia were in accurate or acceptable zones. The mean differences between the measured subcutaneous glucose and the actual plasma glucose concentration were -0.06-3.3 mmol/l (hyperglycemia), and -0.6-1.1 mmol/l (hypoglycemia).
By combining open-flow microperfusion, glucose sensor, and conductivity measurement, glucose concentration in the subcutaneous adipose tissue can be monitored on-line, extracorporeally, and continuously without any in vivo calibration, and gives accurate measurements during hyper- and hypoglycemia.
评估一种用于皮下脂肪组织在线连续葡萄糖测量的新技术，并研究其检测低血糖的准确性。
该方法结合了使用双腔导管对皮下脂肪组织进行的开放流微灌注和体外传感器细胞。等渗无离子溶液通过导管的内腔灌注，与皮下组织液平衡，并通过外腔采样。将测量的采样液电导率与皮下组织液电导率（假设在25℃时恒定值为1.28 S/m）之比作为回收率进行连续监测。在高血糖葡萄糖负荷（n = 8）、低血糖高胰岛素钳夹（n = 6）和24小时监测期（n = 7）期间，根据采样液中测量的葡萄糖和测量的回收率在线计算健康志愿者的葡萄糖浓度。
空腹状态下皮下葡萄糖浓度为动脉化静脉样本中血浆葡萄糖浓度的94%。根据误差网格分析，高血糖期间96.9%的在线测量皮下葡萄糖浓度和低血糖期间96.3%的在线测量皮下葡萄糖浓度处于准确或可接受区域。测量的皮下葡萄糖与实际血浆葡萄糖浓度之间的平均差异在高血糖时为-0.06 - 3.3 mmol/l，在低血糖时为-0.6 - 1.1 mmol/l。
通过结合开放流微灌注、葡萄糖传感器和电导率测量，可以在体外对皮下脂肪组织中的葡萄糖浓度进行在线连续监测，无需任何体内校准，并且在高血糖和低血糖期间均能给出准确测量结果。
