Schaupp L, Ellmerer M, Brunner G A, Wutte A, Sendlhofer G, Trajanoski Z, Skrabal F, Pieber T R, Wach P
Department of Biophysics, Institute of Biomedical Engineering, Graz University of Technology, A-8010 Graz, Austria.
Am J Physiol. 1999 Feb;276(2):E401-8. doi: 10.1152/ajpendo.1999.276.2.E401.
To gain direct access to the interstitial fluid (ISF), a new technique called open-flow microperfusion has been evaluated. This method is based on a double-lumen catheter with macroscopic (0.3-0.5 mm diameter) perforations that is inserted into the subcutaneous adipose tissue and constantly perfused. Thus partial equilibration between the ISF and the perfusion fluid occurs. The glucose concentration of the ISF was determined by established (zero flow rate, no net flux, and recirculation procedures) and new (ionic reference and suction technique) calibration methods by use of open-flow microperfusion. The data show that 1) the glucose concentration in the ISF is significantly lower than the corresponding arterialized venous plasma values during basal steady-state conditions (adipose tissue 3.2 +/- 0.10 mM, plasma 5.27 +/- 0.12 mM) as well as during hyperglycemic clamp experiments (adipose tissue 7.3 +/- 0.13 mM, plasma 9.91 +/- 0.16 mM), and 2) it is possible to determine the recovery continuously by using the ion concentration of the ISF as an internal standard (ionic reference).
为了直接获取组织间液（ISF），一种名为开放流动微灌注的新技术已得到评估。该方法基于一根带有宏观（直径0.3 - 0.5毫米）穿孔的双腔导管，将其插入皮下脂肪组织并持续灌注。这样，ISF与灌注液之间就会发生部分平衡。通过使用开放流动微灌注，采用既定的（零流速、无净通量和再循环程序）以及新的（离子参考和抽吸技术）校准方法来测定ISF的葡萄糖浓度。数据表明：1）在基础稳态条件下（脂肪组织3.2±0.10毫摩尔/升，血浆5.27±0.12毫摩尔/升）以及高血糖钳夹实验期间（脂肪组织7.3±0.13毫摩尔/升，血浆9.91±0.16毫摩尔/升），ISF中的葡萄糖浓度显著低于相应的动脉化静脉血浆值；2）通过将ISF的离子浓度用作内标（离子参考），可以连续测定回收率。
