School of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Anal Bioanal Chem. 2014 Jan;406(3):831-9. doi: 10.1007/s00216-013-7499-4. Epub 2013 Nov 21.
Compounds with strong intramolecular hydrogen bonds (e.g., salicylic acid) have weak intermolecular hydrogen bonding interactions between them and functional monomers in the imprinting process. Consequently, the corresponding molecularly imprinted polymers (MIPs) have no specific adsorption ability. Here, the first magnetic dummy MIPs (MDMIPs) based on benzonic acid as dummy template are successfully developed and evaluated with respect to the applications in selective enrichment and analysis of salicylic acid from complex mixtures. Various parameters affecting absorption/desorption were evaluated for achieving optimal recovery and reducing nonspecific interactions. The prepared MDMIPs showed high adsorption capacity, good selectivity, rapid kinetic binding (40 min) and magnetic separation (5 s), high reproducibility (RSD< 4 % for batch-to-batch evaluation), and stability (only 4 % decrease after 6 cycles). Owing to the efficacy in specific binding and removal of interference, trace level salicylic acid was quantified (0.2 μg/g of fresh mass) in Actinidia chinensis by high-performance liquid chromatography.
具有强分子内氢键的化合物（例如水杨酸）在印迹过程中它们彼此之间以及与功能单体之间具有较弱的分子间氢键相互作用。因此，相应的分子印迹聚合物（MIP）没有特定的吸附能力。在这里，首次成功开发了基于苯甲酸的磁性虚拟分子印迹聚合物（MDMIP），并将其作为虚拟模板，用于从复杂混合物中选择性富集和分析水杨酸的应用。评估了各种影响吸收/解吸的参数，以实现最佳的回收和减少非特异性相互作用。所制备的 MDMIP 表现出高吸附容量、良好的选择性、快速的动力学结合（40 min）和磁分离（5 s）、高重现性（批间评估的 RSD<4%）和稳定性（6 个循环后仅下降 4%）。由于具有特异性结合和去除干扰的功效，通过高效液相色谱法在猕猴桃中定量测定了痕量水平的水杨酸（新鲜质量的 0.2 μg/g）。
