Zhou Can, Chen Jianxiong, Dang Xueping, Ma Xiwen, Ai Youhong, Huang Jianlin, Chen Huaixia
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University.
Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment.
Anal Sci. 2018;34(7):823-829. doi: 10.2116/analsci.18P025.
A new combination between selective polymer monolith microextraction (PMME) and sensitive differential pulse voltammetry (DPV) was developed for the determination of the phytohormone salicylic acid (SA) in Actinidia chinensis. A molecularly imprinted monolithic column (MIMC) thermally in-situ polymerized in a micropipette tip by using SA as a template, 4-vinyl pyridine (4-VP) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker in the mixed porogen of toluene and dodecanol, was employed for the microextraction of SA. The prepared MIMC was characterized by a Fourier transform infrared spectrometer (FI-TR), scanning electron microscope (SEM) and thermo gravimetric analysis (TGA). The results confirmed the binary continuous structure of the porous network. The extracted SA was determined by DPV on a graphene oxide (GO) modified electrode. The joint conditions between MIMC and DPV were investigated practically. Under the optimum conditions, SA could be determined selectively and sensitively in a linear range from 0.1 to 60.0 μg g. The limit of detection was 0.03 μg g and the recoveries were between 86.2 and 105.2%. The proposed joint method was successfully used to determine SA in Actinidia chinensis.
开发了一种选择性聚合物整体微萃取（PMME）与灵敏差分脉冲伏安法（DPV）相结合的新方法，用于测定中华猕猴桃中的植物激素水杨酸（SA）。以SA为模板、4-乙烯基吡啶（4-VP）为功能单体、乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，在甲苯和十二烷醇的混合致孔剂中，通过热原位聚合在微量移液器尖端制备了分子印迹整体柱（MIMC），用于SA的微萃取。采用傅里叶变换红外光谱仪（FI-TR）、扫描电子显微镜（SEM）和热重分析（TGA）对制备的MIMC进行了表征。结果证实了多孔网络的二元连续结构。通过在氧化石墨烯（GO）修饰电极上的DPV测定萃取的SA。实际研究了MIMC与DPV之间的联用条件。在最佳条件下，SA能够在0.1至60.0 μg g的线性范围内被选择性且灵敏地测定。检测限为0.03 μg g，回收率在86.2%至105.2%之间。所提出的联用方法成功用于测定中华猕猴桃中的SA。
