Zhao Wen-Rui, Kang Tian-Fang, Lu Li-Ping, Cheng Shui-Yuan
Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology Beijing 100124 P. R. China
RSC Adv. 2018 Apr 9;8(24):13129-13141. doi: 10.1039/c8ra01250d.
Diethylstilbestrol (DES) is considered a representative example of an exogenous endocrine disrupting compound (EDC). It can retard development in infants, lead to serious metabolic regulation disorders, and even result in distortion and cancer in the reproductive system. Therefore, achieving rapid and accurate analysis of trace amounts of DES in complex environments is of great importance to human health and for environmental protection. Novel magnetic molecularly imprinted polymers (MIPs) with excellent molecular recognition ability and super water-compatibility were developed for the selective capture of DES in water samples. FeO@SiO magnetic nanoparticles (NPs) were synthesized and used as support cores. Molecularly imprinted poly(3-aminophenylboronic acid) (poly(APBA)), synthesized on magnetic cores based on a surface-imprinting strategy, can preferentially bind DES molecules in water samples. The magnetic core-shell MIPs (denoted as FeO@SiO@APBA/MIPs) exhibited high binding capacity and favorable recognition specificity for DES in water. The adsorption kinetics and experimental isotherm data of DES on magnetic MIPs can be well described by the pseudo-second-order kinetic model and the Langmuir isotherm, respectively. The imprinted nanoparticles were subjected to magnetic solid-phase extraction (MSPE) of DES from water samples. The DES content in the samples was determined by high-performance liquid chromatography (HPLC). The peak area increased linearly with increasing DES concentration over the range 0.08-150 μg L, with a detection limit of 0.03 μg L. The recoveries for spiked lake water samples were in the range 97.1-103.2%, with relative standard deviation (RSD) of 2.8-4.3% ( = 6).
己烯雌酚（DES）被认为是外源性内分泌干扰化合物（EDC）的一个典型例子。它会延缓婴儿发育，导致严重的代谢调节紊乱，甚至会使生殖系统出现畸变和癌症。因此，实现对复杂环境中痕量DES的快速准确分析对人类健康和环境保护至关重要。为选择性捕获水样中的DES，开发了具有优异分子识别能力和超强水相容性的新型磁性分子印迹聚合物（MIP）。合成了FeO@SiO磁性纳米颗粒（NPs）并用作支撑核心。基于表面印迹策略在磁芯上合成的分子印迹聚（3-氨基苯硼酸）（聚（APBA））能优先结合水样中的DES分子。磁性核壳MIP（表示为FeO@SiO@APBA/MIP）对水中的DES表现出高结合容量和良好的识别特异性。DES在磁性MIP上的吸附动力学和实验等温线数据分别可以用伪二级动力学模型和朗缪尔等温线很好地描述。对印迹纳米颗粒进行水样中DES的磁性固相萃取（MSPE）。通过高效液相色谱（HPLC）测定样品中的DES含量。在0.08 - 150 μg L范围内，峰面积随DES浓度增加呈线性增加，检测限为0.03 μg L。加标湖水样品的回收率在97.1 - 103.2%范围内，相对标准偏差（RSD）为2.8 - 4.3%（n = 6）。
