Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Sask, Canada.
Planta. 1984 Feb;160(2):168-73. doi: 10.1007/BF00392866.
Wild-type (Ph1) and adenine-requiring (Ad1) cell lines of Datura innoxia Mill. were used in experiments to evaluate arsenate as a growth-lethal compound and its use as a counterselection agent. These experiments led to the devising of methods for the recovery of Ph1 and Ad1 cells after arsenate treatment when plated at low density on feeder plates. The modified counterselection technique was then used to isolate three new auxotrophs from mutagenized suspensions of Ph1 cells, two of which were partially characterized. One, C18, requires casein hydrolysate for growth and lacks an active nitrate reductase; the other, JM3, will grow only when the medium contains threonine.
使用 Datura innoxia Mill. 的野生型（Ph1）和腺嘌呤需求型（Ad1）细胞系进行实验，以评估砷酸盐作为生长致死化合物及其作为反选择剂的用途。这些实验导致了在低密度接种于饲养板上时回收 Ph1 和 Ad1 细胞的方法的设计。然后，将改良的反选择技术用于从 Ph1 细胞的诱变悬浮液中分离出三个新的营养缺陷型，其中两个进行了部分表征。一个是 C18，需要酪蛋白水解物才能生长，并且缺乏活性硝酸还原酶；另一个是 JM3，只有在培养基中含有苏氨酸时才能生长。
