Department of Biology, University of Saskatchewan, S7N OWO, Saskatoon, Sask., Canada.
Planta. 1983 Jan;159(1):12-7. doi: 10.1007/BF00998808.
Cells of adenine(Ad1)- and pantothenate(Pn1)-requiring cells lines ofDatura innoxia Mill. were killed by 2 mM arsenate in the medium when growing but were not killed when in a non-growing state which was induced by starvation for required metabolites. This observation confirmed that arsenate might be useful as a counterselection agent to isolate new auxotrophs from plant cell cultures. Arsenate counterselection using predominantly haploidDatura cell suspensions led to the isolation of a cell line (I-IV) for which isoleucine and valine were both necessary and sufficient for growth. The cell line has been maintained in solid and liquid culture for about three years. The arsenate counterselection technique devised requires improvement. In some experiments, many colonies survived arsenate treatment; in others, there were no survivors. In the former experiments, putative but unstable auxotrophs were regularly observed but all were shown not to be auxotrophic after several retests.
腺嘌呤（Ad1）和泛酸（Pn1）需要的颠茄细胞系的细胞在培养基中被 2mM 砷酸盐杀死，而当处于饥饿状态以去除所需代谢物而不生长时则不会被杀死。这一观察结果证实，砷酸盐可能是一种有用的反选择剂，可以从植物细胞培养物中分离新的营养缺陷型。使用主要是单倍体颠茄细胞悬浮液的砷酸盐反选择导致分离出一种细胞系（I-IV），其生长既需要异亮氨酸又需要缬氨酸。该细胞系已在固体和液体培养中维持了大约三年。设计的砷酸盐反选择技术需要改进。在一些实验中，许多菌落存活了砷酸盐处理；在其他实验中，没有幸存者。在前一种实验中，经常观察到假定但不稳定的营养缺陷型，但经过多次重复试验后，所有这些都证明不是营养缺陷型。
