砷酸盐作为一种潜在的负选择剂，用于培养植物细胞中的缺陷变异体。

Arsenate as a potential negative selection agent for deficiency variants in cultured plant cells.

机构信息

Department of Genetics, Connecticut Agricultural Experiment Station, 06504, New Haven, CT, USA.

出版信息

Planta. 1979 Jan;146(2):155-60. doi: 10.1007/BF00388226.

Abstract

Sodium arsenate is toxic to cultured soybean [Glycine max (L.) Merr.] cells, killing virtually 100% of the cells during a 24-h exposure at a 1-2 mM concentration. However, when growth is previously halted by nitrogen deprivation 50-100% of the cells survive arsenate treatment. Because of this growthdependent toxicity, arsenate has promise as a negative selection agent for cultured plant cells. Using arsenate (2 mM) I was able to select from among 2×10(7) cells a cell line with a growth requirement for an amino acid mixture. This trait was maintained through 9 months of passage but then was lost.

摘要

砷酸钠对培养的大豆（Glycine max（L.）Merr.）细胞有毒，在 1-2 mM 浓度下暴露 24 小时，几乎杀死 100%的细胞。然而，当氮饥饿预先停止生长时，50-100%的细胞在砷酸盐处理下存活下来。由于这种生长依赖性毒性，砷酸盐有望成为培养植物细胞的负选择剂。我使用砷酸钠（2 mM）能够从 2×10（7）个细胞中选择出对氨基酸混合物有生长需求的细胞系。这种特性在 9 个月的传代中得以维持，但随后丢失。