流式细胞术鉴定和分选植物染色体。
Flow-cytometric characterization and sorting of plant chromosomes.
机构信息
Research Institute ITAL, Postbus 48, NL-6700, AA Wageningen, The Netherlands.
出版信息
Theor Appl Genet. 1984 Mar;67(5):463-7. doi: 10.1007/BF00263414.
PMID:24258767
Abstract
Flow cytometric measurements of DNA frequency distribution were used to follow the synchronization process in suspension cells from Haplopappus gracilis (2n=4). Metaphase chromosomes were isolated from these synchronized cells and both the acro- and metacentric chromosomes were sorted by flow cytometry based on the different DNA contents. Possible applications of this procedure in fundamental genetics as well as practical plant breeding are discussed.
摘要
流式细胞术测量 DNA 频率分布被用来跟踪悬浮细胞中海滨犬舌草（2n=4）的同步过程。从中分离出中期染色体，并根据不同的 DNA 含量通过流式细胞术对近端着丝粒和中央着丝粒染色体进行分选。讨论了该程序在基础遗传学和实际植物育种中的可能应用。
Zotero 插件