通过流式细胞术和分选技术对人类染色体进行测量和纯化。
Measurement and purification of human chromosomes by flow cytometry and sorting.
作者信息
Carrano A V, Gray J W, Langlois R G, Burkhart-Schultz K J, Van Dilla M A
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1382-4. doi: 10.1073/pnas.76.3.1382.
Abstract
The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type--namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9--12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.
摘要
利用33258个Hoechst荧光，通过高分辨率流式细胞术将正常二倍体成纤维细胞株的24种人类染色体类型分为15组。与每组相关的染色体通过流式细胞仪分选到显微镜载玻片上，并通过喹吖因显带分析进行鉴定。来自同一细胞株的中期染色体的DNA细胞光度测定法支持并扩展了这一鉴定。纯化的四组是由于单一类型的染色体——即5号、6号、13号和17号染色体。另外八组也被分离出来，发现包含以下染色体：1号和2号；3号和4号；7号、8号和X染色体；9号至12号；14号和15号；16号和18号；20号和Y染色体；以及19号、21号和22号。12个分选组分的平均纯度为78%。
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