提高少叶补血草（Nutt）灰色自由细胞悬浮液电镀效率的条件。
Conditions for a high plating efficiency of free cell suspensions of Haplopappus gracilis (Nutt) gray.
机构信息
Radiation Biophysics Group, Association Euratom - ITAL Wageningen, the Netherlands.
出版信息
Theor Appl Genet. 1978 Jul;51(4):161-7. doi: 10.1007/BF00273141.
Suspensions of Haplopappus gracilis cells, containing about 80% free cells, were obt ained from log-phase cultures by filtration through 3 nylon sieves having decreasing mesh widths from 297, 210 and 88 μm. From the free cell suspensions, 75 to 90% of the cells developed into visible colonies when the plating procedure was divided into two steps: a) plating the cells at high concentration in soft agar on feeder agar; b) replating the resulting aggregations at appropriate concentrations on fresh feeder agar. From the results, it is inferred that, in the replating step, the volume of the inoculum is the deciding factor which influences the resulting plating efficiency.
通过 3 张尼龙筛网（网眼宽度逐渐变窄，分别为 297、210 和 88μm）对处于对数生长期的哈氏旋覆花细胞进行过滤，获得了含有约 80%游离细胞的悬浮液。从游离细胞悬浮液中，当接种程序分为两步进行时，75%至 90%的细胞会形成可见的菌落：a）在饲养琼脂上的软琼脂中以高浓度接种细胞；b）在新鲜的饲养琼脂上以适当的浓度重新接种得到的聚集物。根据这些结果可以推断，在重新接种步骤中，接种物的体积是影响最终接种效率的决定性因素。
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