Department of Botany, University of Durham, DH1 3LE, Durham, UK.
Planta. 1984 May;160(6):559-68. doi: 10.1007/BF00411145.
Two types of storage protein, vicillin and legumin, occur in the developing pea seed. Storage-protein gene expression has been studied during cotyledon development by assaying specific transcripts produced by nuclei isolated at different stages, and from pea leaves. The proportion of vicilin to legumin transcripts changed during development: vicilin transcripts predominated at 9 and 11 days after flower opening (d.a.f.) and were similar in amount to legumin at 14 d.a.f., whereas at 18 d.a.f., legumin transcripts predominated and little vicilin transcription was observed. The rate of storage-protein transcription correlated with previously determined (Gatehouse et al. 1982) mRNA levels during seed development; these transcripts were not detected in similar assays using leaf nuclei. Transcription by cotyledonary nuclei for short times indicated that post-transcriptional processing may be a factor in regulating mRNA levels, at least in the earlier part of seed development.
两种贮藏蛋白，豆球蛋白和伴豆球蛋白，存在于发育中的豌豆种子中。通过测定在不同阶段分离的核产生的特定转录本，并从豌豆叶片中，研究了贮藏蛋白基因在子叶发育过程中的表达。豆球蛋白和伴豆球蛋白转录本的比例在发育过程中发生变化：在开花后 9 天和 11 天（d.a.f.），豆球蛋白转录本占优势，与 14 天 d.a.f.时的豆球蛋白转录本数量相似，而在 18 天 d.a.f.时，豆球蛋白转录本占优势，观察到很少的豆球蛋白转录。贮藏蛋白转录的速度与种子发育过程中先前确定的（Gatehouse 等人，1982 年）mRNA 水平相关；在使用叶片核进行的类似测定中，未检测到这些转录本。短时间的子叶核转录表明，至少在种子发育的早期阶段，转录后加工可能是调节 mRNA 水平的一个因素。
