缺硫豌豆种子中贮藏蛋白基因表达的转录和转录后调控
Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds.
作者信息
Beach L R, Spencer D, Randall P J, Higgins T J
出版信息
Nucleic Acids Res. 1985 Feb 11;13(3):999-1013. doi: 10.1093/nar/13.3.999.
Abstract
We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S deficiency, transcription of legumin genes increased by two-fold whereas the level of legumin mRNA increased 20-fold in the same period. Vicilin gene transcription decreased during recovery, consistent with a gradual decrease in mRNA levels. We conclude that regulation of legumin mRNA levels in response to changing S status is post-transcriptional, whereas that of vicilin mRNA is transcriptional. In contrast, during normal plant development the expression of both of these gene families appears to be under transcriptional control.
摘要
我们利用离体豌豆细胞核中的体外转录来确定种子贮藏蛋白豆球蛋白和豌豆球蛋白基因的表达是在转录水平还是转录后水平受到调控，以响应发育中种子硫状态的变化。在从硫缺乏状态恢复的最初48小时内，豆球蛋白基因的转录增加了两倍，而同期豆球蛋白mRNA的水平增加了20倍。豌豆球蛋白基因转录在恢复过程中下降，这与mRNA水平的逐渐降低一致。我们得出结论，响应硫状态变化时豆球蛋白mRNA水平的调控是在转录后水平，而豌豆球蛋白mRNA的调控是在转录水平。相比之下，在正常植物发育过程中，这两个基因家族的表达似乎都受到转录控制。
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本文引用的文献
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