Shirsat A H
Department of Botany, Durham University, UK.
Mol Gen Genet. 1988 Apr;212(1):129-33. doi: 10.1007/BF00322455.
The legumin storage proteins of Pisum sativum are coded for by a multigene family. An insertion element (Pis1) has been found integrated into the 5' flanking sequence of the legC legumin seed storage protein gene. This element contains all the sequence features of the CACTA family of insertion elements, has perfect 12 bp inverted repeats at its termini, and generates a target host site duplication upon integration. An 8 bp sequence within the left arm of the insertion element shows perfect homology to a sequence in the legC flanking region. Three stem-loop structures which can be formed within the element have the same stem sequence.
豌豆的豆球蛋白贮藏蛋白由一个多基因家族编码。已发现一个插入元件(Pis1)整合到legC豆球蛋白种子贮藏蛋白基因的5'侧翼序列中。该元件包含CACTA插入元件家族的所有序列特征,在其末端有完美的12 bp反向重复序列,并在整合时产生靶宿主位点重复。插入元件左臂内的一个8 bp序列与legC侧翼区域的一个序列具有完全同源性。该元件内可形成的三个茎环结构具有相同的茎序列。