Department of Plant Genetics, The Weizmann Institute of Science, 76100, Rehovot, Israel.
Theor Appl Genet. 1984 Apr;67(6):499-504. doi: 10.1007/BF00264893.
Cybrid plants having the nuclear genomes of one species and either or both plastomes and chondriomes of another species were obtained by fusing protoplasts of Nicotiana sylvestris, as "recipients", with X-irradiated protoplasts of N. rustica as "donors" of chloroplasts and mitochondria. Forty-nine flowering plants, derived from 28 calli, were analysed. As expected, they all had N. sylvestris (i.e. "recipients") morphology. Chloroplast DNA restriction patterns indicated that 8 and 41 plants had N. rustica and N. sylvestris plastomes, respectively. Some of the plants with either type of plastomes produced sterile pollen but none showed anther malformation typical to alloplasmic male sterility. Chondriome identification by mitochondrial DNA restriction analysis of cybrid plants revealed only restriction patterns which were either similar or identical to those of N. sylvestris while no cybrids with N. rustica restriction patterns were detected.
通过融合作为“受体”的野生烟草原生质体与作为叶绿体和线粒体“供体”的 X 射线照射的粗糙烟草原生质体，获得了具有一个物种核基因组和另一个物种的一个或两个质体基因组和线粒体基因组的杂交植物。分析了来自 28 个愈伤组织的 49 株开花植物。正如预期的那样，它们都具有野生烟草（即“受体”）的形态。叶绿体 DNA 限制图谱表明，有 8 株和 41 株植物分别具有粗糙烟草和野生烟草的质体。一些具有任一种质体的植物产生不育花粉，但没有一种植物表现出所有雄性不育特有的花药畸形。通过对杂交植物的线粒体 DNA 限制分析进行的线粒体鉴定表明，只有与野生烟草相似或相同的限制图谱，而没有检测到具有粗糙烟草限制图谱的杂交植物。
