Department of Plant Genetics, Weizmann Institute of Science, 76100, Rehovot, Israel.
Theor Appl Genet. 1984 Apr;67(6):491-7. doi: 10.1007/BF00264891.
Protoplasts of a mutant line of Nicotiana tabacum having a maternally-transmitted chlorophyll deficiency were fused with protoplasts of two alloplasmic-male-sterile Nicotiana lines by the "donor-recipient" technique. In both fusion experiments variegated plantlets were regenerated which were shown to contain cytoplasms of mixed chloroplast nature. This confirms that with the "donor-recipient" method one can obtain mixed cytoplasms of genetically different chloroplasts. We present a convenient system to assay for genetic recombination between chloroplasts by combining use of several cytoplasmic markers: vis. chlorophyll pigmentation, chloroplast DNA restriction patterns, tentoxin resistance and male sterility. Within the limits of the experiment no recombinant types were recovered.
用“供体-受体”技术将带有母系遗传叶绿素缺乏的烟草突变体原生质体与两个叶绿体雄性不育烟草异质系的原生质体融合。在这两个融合实验中，再生了斑驳的植物，它们被证明含有混合叶绿体性质的细胞质。这证实了用“供体-受体”方法可以获得遗传上不同的叶绿体的混合细胞质。我们提出了一个方便的系统来检测叶绿体之间的遗传重组，方法是结合使用几种细胞质标记：叶绿素色素、叶绿体 DNA 限制图谱、 Tentoxin 抗性和雄性不育。在实验的范围内，没有回收重组类型。
