Perl A, Aviv D, Galun E
Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel.
Mol Gen Genet. 1991 Jan;225(1):11-6. doi: 10.1007/BF00282636.
We have successfully bridged the intergeneric barriers between Nicotiana and Solanum with respect to chondriome transfer. To enable this transfer we utilized the donor-recipient protoplast-fusion procedure. Consequently protoplasts of a Nicotiana sylvestris line with putatively oligomycin-resistant mitochondria (line OliR38) were used as irradiated chondriome donors and iodoacetate-treated protoplasts of Solanum tuberosum cv. Desiree served as recipients. The plated fusion products as well as their derived colonies and calli were exposed to gradually increasing levels of oligomycin. The resulting plantlets had potato morphology and were analyzed with respect to their mitochondrial DNA and chloroplast DNA. Fifteen out of 50 regenerated plants were verified as true cybrids. Detailed analyses of one cybrid revealed chondriome components from the oligomycin-resistant donor line, OliR38, but retention of the plastome of potato. This cybrid was oligomycin-resistant as revealed by root-culture analysis. It was thus verified that due to selection, chondriome components could be transferred from a N. sylvestris donor into a cybrid having all the phenotypic features controlled by the nucleus of the recipient fusion partner (S. tuberosum).
我们已成功跨越了烟草属和茄属之间关于线粒体基因组转移的属间障碍。为实现这种转移，我们采用了供体 - 受体原生质体融合程序。因此，具有假定抗寡霉素线粒体的烟草野生种品系（OliR38品系）的原生质体被用作经辐射的线粒体基因组供体，而碘乙酸处理的马铃薯栽培种Desiree的原生质体用作受体。将平板培养的融合产物及其衍生的菌落和愈伤组织暴露于逐渐增加水平的寡霉素中。所得的植株具有马铃薯形态，并对其线粒体DNA和叶绿体DNA进行了分析。50株再生植株中有15株被确认为真正的胞质杂种。对一个胞质杂种的详细分析表明，其线粒体基因组成分来自抗寡霉素的供体品系OliR38，但保留了马铃薯的质体基因组。通过根培养分析表明，这个胞质杂种具有抗寡霉素特性。因此证实，通过选择，线粒体基因组成分可以从烟草野生种供体转移到一个具有由受体融合伙伴（马铃薯）细胞核控制的所有表型特征的胞质杂种中。
