Departamento de Genética, Facultad de Biología, Universidad Complutense, Madrid 3, Spain.
Theor Appl Genet. 1984 May;68(1-2):75-80. doi: 10.1007/BF00252316.
The nucleolar organizer activity of the Agropyron elongatum, its amphiploid with hexaploid wheat (Triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. Four Ag-NORs are observed in A. elongatum corresponding to the chromosomes 6E and 7E. In the amphiploid T. aestivum - A. elongatum, eight Ag-NORs are observed which corresponds the wheat chromosomes 1B and 6B and to the elongatum chromosomes 6E and 7E. Thus, there is codominance in the nucleolar organizer activity of the chromosomes of the two species. However, a partial amphiplasty is detected since less than 8 Ag-NORs (7 up to 4) are observed in some metaphase cells; the chromosomes 6E and 7E are occasionally suppressed by wheat chromosomes. This conclusion is confirmed by the behaviour of the addition lines since only in those corresponding to the chromosomes 6E and 7E are the elongatum chromosomes nucleolar active although occasionally they can be suppressed by wheat chromosomes.
用银染法分析了长穗偃麦草、长穗偃麦草与六倍体普通小麦的异源双体及其附加系的核仁组织活动。长穗偃麦草有 4 个 Ag-NORs，对应着 6E 和 7E 染色体。在异源双体小麦-长穗偃麦草中，观察到 8 个 Ag-NORs，对应着小麦的 1B 和 6B 染色体以及长穗偃麦草的 6E 和 7E 染色体。因此，两种物种的染色体的核仁组织活动具有共显性。然而，由于在一些中期细胞中观察到的 Ag-NORs 数（7 个到 4 个）少于 8 个，因此检测到部分异源性；6E 和 7E 染色体偶尔被小麦染色体抑制。这一结论通过附加系的行为得到证实，因为只有在对应着 6E 和 7E 染色体的那些附加系中，长穗偃麦草染色体才具有核仁活性，尽管它们偶尔会被小麦染色体抑制。
