Departamento de Genética, Facultad de Biologia, Universidad Complutense, 3, Madrid, Spain.
Theor Appl Genet. 1984 Jan;67(2-3):207-13. doi: 10.1007/BF00317037.
Amphiplasty in hexaploid triticale, the artificial amphiploid of tetraploid wheat and diploid rye, is analyzed for the first time using a modified, highly reproducible, silver-staining procedure. A comparative analysis of metaphase somatic cells by phase contrast, C-banding and silver-staining of the hexaploid triticale cv. 'Cachirulo' and its parents, namely, the tetraploid durum wheat cv. 'Enano de Andujar' and the diploid rye cv. 'Petkus' has been made. Two silver-stained nucleolar organizer regions (Ag-NORs) (the chromosome pair 1 R) are observed in all rye plants analyzed, whereas four Ag-NORs (chromosome pairs 1 B and 6 B) are found both in the tetraploid wheat parent and in the triticale. The rye Ag-NORs are absent in the triticale. Since the Agstaining reaction of NORs can be considered as an indication for genetic activity, the silver procedure can be used to visualize gene functionality at the rDNA sites with conventional light microscopy and, consequently, the modified Ag-staining method described can be very useful in analyzing the amphiplasty phenomenon in natural or artificial hybrid combinations and derivatives in the Triticum group and its relatives.
首次使用改良的、高度重现性的银染程序分析了六倍体小黑麦（四倍体普通小麦和二倍体黑麦的人工异源多倍体）的两栖现象。通过相差、C-带和银染对比分析了六倍体小黑麦品种‘Cachirulo’及其亲本，即四倍体硬质小麦品种‘Enano de Andujar’和二倍体黑麦品种‘Petkus’的中期体细胞。在所有分析的黑麦植物中都观察到了两个银染核仁组织区（Ag-NORs）（第 1R 染色体对），而在四倍体小麦亲本和小黑麦中都发现了四个 Ag-NORs（第 1B 和 6B 染色体对）。小黑麦中没有黑麦的 Ag-NORs。由于 NORs 的银染反应可以被认为是遗传活性的指标，因此可以使用银染程序在常规光学显微镜下可视化 rDNA 位点的基因功能，因此，所描述的改良 Ag 染色方法在分析自然或人工杂交组合及其衍生物中的两栖现象在小麦族及其近缘植物中非常有用。
