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C-带多态性与绒毛山羊草(L.)坎达尔吉的核仁活性分析,及其附加到六倍体小麦和双二倍体 Triticum dicoccum - D. villosum 中的染色体。

C-banding polymorphism and the analysis of nucleolar activity in Dasypyrum villosum (L.) Candargy, its added chromosomes to hexaploid wheat and the amphiploid Triticum dicoccum - D. villosum.

机构信息

Institut für Pflanzenbau und Pflanzenzüchtung, Technische Universität München, D-8050, Freising-Weihenstephan, Germany.

出版信息

Theor Appl Genet. 1987 Jan;73(3):337-42. doi: 10.1007/BF00262498.

DOI:10.1007/BF00262498
PMID:24240992
Abstract

C-banding patterns and nucleolar activity were analyzed in Dasypyrum villosum, its added chromosomes to hexaploid wheat and the hexaploid amphiploid Triticum dicoccum-D. villosum. Two different populations of the allogamous species D. villosum (2n= 14, VV) from Greece and Italy were analyzed showing a similar polymorphism for C-banding pattern. Six of the seven addition lines were identified by their characteristic C-banding pattern. No polymorphism between both members of each added alien chromosome was found. Furthermore, nucleolar activity and competition were studied by using silver staining procedure. In D. villosum only one chromosome pair, A, was found to be responsible for organizing nucleoli. The results obtained in the amphiploid and in the addition lines demonstrate that nucleolar activity is restricted to SAT-chromosomes 1B and 6B of wheat, while those of D. villosum remain inactive.

摘要

在长穗偃麦草、其添加到六倍体小麦的染色体和六倍体双二倍体 Triticum dicoccum-D. villosum 中分析了 C-带模式和核仁活性。分析了来自希腊和意大利的两个不同的异源种长穗偃麦草(2n=14,VV)种群,显示出 C-带模式的相似多态性。通过其特征性的 C-带模式鉴定了 7 个添加系中的 6 个。在每个添加的外源染色体的两个成员之间没有发现多态性。此外,还通过银染程序研究了核仁活性和竞争。在长穗偃麦草中,仅发现一对染色体 A 负责组织核仁。在双二倍体和添加系中获得的结果表明,核仁活性仅限于小麦的 SAT 染色体 1B 和 6B,而长穗偃麦草的核仁仍然不活跃。

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Molecular and Cytogenetic Characterization of New Wheat-Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit.

本文引用的文献

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Morphology and chromosome pairing of a hybrid between Triticum durum Desf. and Haynaldia villosa (L.) Schur.硬粒小麦与长穗偃麦草杂种的形态和染色体配对
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具有收获后再生长习性的新型小麦-短柄披碱草衍生物的分子和细胞遗传学特征
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Theor Appl Genet. 1988 Oct;76(4):513-29. doi: 10.1007/BF00260901.
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Theor Appl Genet. 1991 Mar;81(3):363-8. doi: 10.1007/BF00228677.
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Restriction fragment patterns of chloroplast and mitochondrial DNA of Dasypyvum villosum (L.) candargy and wheats.短柄草(Dasypyrum villosum (L.) Candargy)叶绿体和线粒体 DNA 的限制性片段模式与小麦。
Theor Appl Genet. 1993 Oct;87(1-2):44-8. doi: 10.1007/BF00223742.
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Thinopyrum distichum chromosome morphology and C-band distribution.节节麦染色体形态和 C 带分布。
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8
Isolation, characterization and application of a species-specific repeated sequence from Haynaldia villosa.从节节麦中分离、鉴定和应用一个种特异性重复序列。
Theor Appl Genet. 1995 Mar;90(3-4):526-33. doi: 10.1007/BF00221999.
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Identification of Haynaldia villosa chromosomes added to wheat using a sequential C-banding and genomic in situ hybridization technique.利用连续 C 带和基因组原位杂交技术鉴定添加到小麦中的节节麦染色体。
Theor Appl Genet. 1996 Jan;92(1):116-20. doi: 10.1007/BF00222960.
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A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat.一个新的罗伯逊易位事件导致携带对 Ug99 有效抗性基因 Sr52 的节节麦被转移到普通小麦中。
Theor Appl Genet. 2011 Jun;123(1):159-67. doi: 10.1007/s00122-011-1574-z. Epub 2011 Mar 25.
通过银染程序在黑小麦中证明小麦-黑麦核仁竞争(变构)。
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