Department of Agricultural Chemistry, Kyoto University, 606, Kyoto, Japan.
Plant Cell Rep. 1982 Apr;1(3):101-3. doi: 10.1007/BF00272363.
Tests for calluses rich in tropane alkaloids were made with newly induced calluses of Atropa belladonna, Datura stramonium and Hyoscyamus niger. Only calluses of H. niger gave an alkaloid-positive test.A Hyoscyamus cell line had the highest total alkaloid content of all the calluses screened by the cell-squash alkaloid assay. Both hyoscyamine and scopolamine were identified in the cultured cells of this line by TLC, GLC and GC-MS.
用颠茄、曼陀罗和天仙子的新诱导愈伤组织进行了富含托烷生物碱愈伤组织的测试。只有天仙子愈伤组织呈生物碱阳性反应。通过细胞压碎生物碱测定法筛选出的所有愈伤组织中，Hyoscyamus 细胞系的总生物碱含量最高。通过 TLC、GLC 和 GC-MS 在该细胞系的培养细胞中鉴定出了莨菪碱和东莨菪碱。
